การจำแนกเอคโตไมคอร์ไรซาของไม้วงศ์ยางในป่าเต็งรังโดยใช้ลำดับเบสของ Internal Transcribed Spacer (ITS) และ Mitochondrial Large Subunit Ribosomal DNA

Identification of Ectomycorrhizal Fungi of Dipterocarpaceae in Dry Dipterocarp Forests Based on Sequences of Internal Transcribed Spacer (ITS) and Mitochondria] Large Subunit Ribosomal DNA

Name: ทัศนีย์ ยุทธสิทธิ์โยธิน

Organization : มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

keyword: Dipterocarpacene

; Dry dipterocarp forests

; Dipterocarpacene

; Ectomycorrhizal fungi

; Mitochondria1 large subunit ribosomal

; Internal transcribed spacer

Abstract: The family Diprerocopoceae forms a doininant family in tropical forests. Since thestructures of the plants are uniform and their wood is hard, inany species have been exploited ingeneral construction. In the last few decades, dipterocarp forests had bcen overlogged anddegraded. thus planting diptcrocarps has gained much attention. Dipterocarps are unusual amongtropical trees in being associated with ectomycorrhizal fungi. Ectomycorrhizal fungi are themutually beneficial symbioses of fungi and fine roots, and are responsible for the uptake ofnutrients that support the tree growth. The potential use of ectoinycorrhiza in reforestationdepends upon the kno~dedge of biological diversity and symbiotic range between rhese twoorganisms. Therefore, the species of ectoinycorrhizal fungi in the forest have essentially beenidentified. Prior to development of molecular techniques, the study of symbiotic relationshipbetween these two organisms was difficult because of no simple way to identify each species.Molecular methods halve facilitated this study. In this work, indegenous symbiotic fungi inectomycorrhizae in dry dipterocarp forests were identified by amplification of internaltranscribed spacer (ITS) consisting of ITS1, 5.8SrDNA, and ITS2 and a portion of mitochondria1large subunit of ribosomal DNA (mt LrDNA) using PCR technique. The sequences of the PCRproducts were evaluated to identify the genera of ectomycorrhizae. Thirty eight samples ofectomycorrhizal roots were collected from three dipterocarp forests in the north and north-east ofThailand, i.e., Queen Sirikit Botanic Garden in Chiangmai province, Sarawin Natural Park inMaehongson province, Sakaerat Ensironmental Research Center, and Taknrt Rang mountain inNakornratchasrima province. Phylogenetic analyses and comparison of the DNA sequences fromectolnycorrhizae and mushrooms (both in collections and available databases) were performed toidentify mycorrhizal fungi. The results revealed that the most abundant of fungi were in groupThelephoroid (Thelephora spp. and Tomentella spp.) and Family Russulaceae (Russula spp.,Lactarius spp., and Gymnomyces sp.). Some species of these fungi showed broad host range anddistributed in all three dry dipterocarp forests. Only few species of Family CortinareaceneEctomycorrhizal fungi / Dipterocarpacene / Dry dipterocarp forests /Internal transcribed spacer / Mitochondria1 large subunit ribosomalDNA(Cortinaroid and blocj,be spp.) and Family Scler-odermatoceae (Scleroderma sp. and Pisolithusspp.) were also found in some ectomycorrhizal roots.

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: ดร.ทวีรัตน์ วิจิตรสุนทรกุล

Role: อาจารย์ที่ปรึกษา

Created: 2544

Issued: 2005-10-26

Modified: 2006-05-24

วิทยานิพนธ์/Thesis

ISBN: 9745949244

CallNumber: BIT372

tha

DegreeName: วิทยาศาสตรมหาบัณฑิต

Level: ปริญญาโท

Descipline: เทคโนโลยีชีวภาพ

Grantor: มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

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