การปรับปรุงเสถียรภาพของเอ็นไซม์ไซลาเนสทนด่างจาก alkaliphilic bacillus firmus K-1 ต่ออุณหภูมิสูงด้วยสารเคมี

Improvement of Thermal Stability of Alkalinc Xylanase from alkaliphilic bacillus firmus K-1 by Chemicals

Name: กณิฏฐา สิรยานุวัฒนกุล

Organization : มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

keyword: alkaliphilic bacillus firmus K-1

; alkaline xylanase

; thermal stability improvement

; stability improvement by chemicals

Abstract: Thermal stability improvement of xylanasc from Rncillus finntrs K-l by chcmicalmethod was performed in this study. The assays were carried out by chemical addition usingchemical substances including amino acid, proteins, metal ions, hydroxyl group compounds,detergents and mixed chemical substances to the xylanase concentration at 0.118 mg protein/ml,at 55OC, and pH 10.0 and that gave half-life of approximately 3.0 hours. Thc experimentalresults indicated that each chcmical substance could improvc thcrmal stability of xylanase as thehalf-lives of all effective chemical substances added xylanase were detected in the range of 5.0-11.0 hour. The half-lives of the amino acid and proteins added xylanase were found in the rangof 7.0-9.8 hour while tlle half-life of BSA at the concentration of 1.5% (w/v) added xylanase gavethc most effective value at 9.8 hour. The half-lives of the metal ions added xylanase were in therange of 5.0-7.3 hour wlule the half-lives of the mixed chemical substances consistcd of FeSO4,CaCl2 and MgSO4 at the concentrations of 1.0, 5.0 and 100.0 mM rcspcctivcly, addcd xylanasegave the most effective value at 7.5 hour. The hydroxyl group addcd xylanase showed theeffective half-lives in range of 4.3-8.0 hour while thc addition of glyccrol and sucrose at theconcentrations of 5.0% (vlv) and 1.5 M, respectively, gave the same most effective value at 8.0hour. The detergents addcd xylanase showed the effective half-livcs in the range of 4.4-5.8 hourwhen the addition of sodium deoxycholate at the concentration of 1.00% (vlv) and Tween 80 atthe concentration of 0.01% (vlv) gave the most effective same value at 5.8 hour. The mixedchemical substances consisted of three metal ions (FeSO4, CaCI2 and MgSO4 at the concentrationof 1.0, 5.0 and 100.0 mM respectively), glycerol and sucrose showed the effective half-lives inthe range of 9.0-11.0 hour while the addition of mixed chemical substances consisted of threemetal ions, glycerol at the concentration of 5.0% (vlv) and sucrose at thc conccntration of 1.5 Mgave the most effective valne at I 1.0 hour which was 3.7 times higher than the controlOf the resdts as descn'bed above, the mixed chemical substances, which consisted ofthree metal ions (FeSO4, CaCl2 and MgSO4 at the concentration at 1.0, 5.0 and 100.0 mMrespectively), glycerol and sucrose was the most effective chemical addition to improve thcrmalstability of xylanase. Thus, this result shows its possibility in application of thc improvedthermalstable xylanase in pulp and paper industry or another relevant applicable industries.

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: รศ.ดร.กนก รัตนะกนกชัย

Role: อาจารย์ที่ปรึกษา

Created: 2544

Issued: 2005-10-21

Modified: 2019-06-24

วิทยานิพนธ์/Thesis

ISBN: 9746287257

CallNumber: BIT348

tha

DegreeName: วิทยาศาสตรมหาบัณฑิต

Level: ปริญญาโท

Descipline: เทคโนโลยีพลังงาน

Grantor: มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

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