Development of Sucrose Sensors by Electropolymerization Technique

การพัฒนาซูโครสเซนเซอร์โดยเทคนิคอิเล็คโตรโพลีเมอร์ไรเซซั่น

Name: Walaithip Chotiwongpipat

Organization : King Mongkut's Institute of Technology Thonburi

keyword: Electropolymerization

; Sucrose sensor

; Amperometric

Abstract: The objective of this work is the construction of sucrose sensorsusing electropolymerization and cross-linking for immobilization three enzymes,namely, glucose oxidase, mutarotase and invertase on platinum electrode.Glucose oxidase was immobilized by electropolymerization from 30 mM 1,3-DAB and 15 units/ml glucose oxidase in 0.1 M sodium phosphate buffer pH7.0. Poly 1,3-DAB films were formed on platinum electrode at a constantpotential of +650 mV VS SCE for 3 minutes. Seventy-five units/mlmutarotase and 800 units/ml invertase were co-immobilized on the surface ofpoly 1,3-DAB/glucose oxidase electrode by cross-linked with 0.5% BSA and0.05% glutaraldehyde in 0.1 M sodium phosphate buffer pH 5.7 for 90minutes. The measuring system for sucrose was based on an amperometricmethod by detection hydrogen peroxide at the potential +700 mV VS SCE.The operational conditions on measurement of sucrose sensor were 0.1 Msodium phosphate buffer in range of pH 5.0-7.0 and at 25-40 C. However,at 25 C the sucrose sensors were more stable under continuous operation.The sucrose sensors showed the linear range up to 8 mM and a response timewas 1 minute. The activity of sucrose sensor remained at 90% of its initialactivity after 80 uses. The shelf life time was over 100 days.The linearity of sucrose sensors could be extended up to 18 mM ifcovered with a dialysis membrane. The response time, however, was longerto 2 minutes. Preparation of sucrose sensors by electropolymerization andcross-linking gave an advantage over the PCP-enzyme electrode.Interference from ascorbic acid was eliminated. Ten sucrose sensors preparedby electroplymerization and cross-linking showed good reproducibility. Theresponse to sucrose was 6.5 +/- 0.35 UA.

King Mongkut's Institute of Technology Thonburi

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: Dr. Morakot Tanticharoen

Role: Supervisor

Name: Sopa Klinchan

Role: Supervisor

Created: 1994

Issued: 2005-04-12

Modified: 2006-05-30

วิทยานิพนธ์/Thesis

ISBN: 9746213954

CallNumber: BIT41

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biotechnology

Grantor: King Mongkut's University of Technology Thonburi

©copyrights King Mongkut's University of Technology Thonburi

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	BIT41.pdf	2.31 MB	27	2025-12-11 10:01:46
2	BIT41ab.pdf	109.12 KB	10	2018-05-13 01:48:23
3	BIT41ab.smi	2.02 KB	7	2018-05-13 01:48:24

ใช้เวลา

0.041857 วินาที