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Pan Ton. Immunological and pathological studies in mice resistance to murine malaria. Master's Degree(Pathobiology). Mahidol University. Mahidol University Library and Knowledge Center. : Mahidol University, 1988.
Immunological and pathological studies in mice resistance to murine malaria
การศึกษาภูมิคุ้มกัน และพยาธิสภาพในหนูที่มีความต้านทานต่อเชื้อมาลาเรีย
Name: Pan Ton
MeSH:
Antibody Formation
MeSH:
Immunity, Cellular
MeSH:
Immunoblotting
MeSH:
Malaria
-- immunology
Abstract:
Immunoblotting and immunofluorescent technipues were used to study immunological reaction between antibody in sera of experimental mice during acute infectiona and after resovery from P. berghei and P. chabaudi infections. Malarial parasite antigens of schizont infected erythorcytes were sonicated and solubilized in the presence of dithiothreitol before electropeparation on sodium dodecyl sulphate polyacrylamide gel. After electrotransfer to nitrocellulose paper, the separated protein components were reacted with individual serum of experimental mouse. Sera from P. berghei-chloroquin cured mice and P. chabaudi self recovered mice showed several prominent reacting bands of polypeptides of P. berghei and P. chabaudi infected erythrocytes on immunnoblots respectively. For P.berghei chloroguin cured mice, their sara reacted with polypeptides in the range of molecular weights 73-180 Kd, whereas sora from P.chabaudi self recovered mice showed reaction with polypeptides in the ranfe of less than 29 Kd-180 Kd. Particularly, the polypeptide with molecular weight 73 Kd was consistently reacted with immune sera from recovered mice. Whereas normal mouse sers showed no reaction with any polypeptides of P. berghei and P. chabaudi infected erythrocytes. The immune sera from recovered mice also did not reacted with protein components of normal mouse erythrocytes. This may indicate that immune sera of recovered mice contained antibodies direct against malaria derived antigens. By indirect immunofluorescent antibody technique, the antibody level was low at the early phase of infection and higher at recovery phsed. There were no significant alterations in livers, kidneys and spleens of recovered mice, except the mononuclear cells infilitation in the interstitial area fo kidney, periportal area of liver and hypertrophy and hyperplasia of white pulp with increasing plasma cell in red pulp of spleen Whereas macrophages containing malarial pigments as small graunles were observed during the patent infection in liver, kidney and spleen and larger forms during recovery phase. These findings may suggest that the elimination of malaria parasites was conferrred by both cellular and humoral immune responses.
Mahidol University. Mahidol University Library and Knowledge Center
Address:
NAKHONPATHOM
Email:
liwww@mahidol.ac.th
Name: Somnate Boonpucknavig
Role:
Thesis Advisors
Name: Wittaya Thammawit
Role:
Thesis Advisors
Name: Galayanee Doungchawee
Role:
Thesis Advisors
Created:
1988
Modified:
2018-04-25
Issued:
2018-04-25
วิทยานิพนธ์/Thesis
application/pdf
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Pathobiology
Grantor: Mahidol University
ใช้เวลา
0.339192 วินาที
Pan Ton
Somnate Boonpucknavig
Wittaya Thammawit
| Title | Creator | Type and Date Create |
|---|---|---|
| Immunological and pathological studies in mice resistance to murine malaria
มหาวิทยาลัยมหิดล Somnate Boonpucknavig;Wittaya Thammawit;Galayanee Doungchawee | Pan Ton | วิทยานิพนธ์/Thesis |
Galayanee Doungchawee