การผลิตน้ำตาลไซโลสจากวัสดุเหลือที้งทางการเกษตรโดยเอ็นไซม์ในถังปฏิกรณ์เยื่อแผ่น

Xylose Production from Agricultural Residues by Enzymatic Hydrolysis in an Ultrafiltrater membrane reactor

Name: ทัศนีย์ เชื้อทอง

Organization : มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

keyword: Hemicellulose

; Xylanase

; B-xylosidase

; Batch reactor

; Hydrolysis

; Membrane reactor

; เฮมิเซลลูโลส

; ไซลาเนส

; เบต้าไซโลซิเดส

Abstract: This research presents the study of enzymatic hydrolysis ofhemicellulose of corn hull to xylose. The enzyme composed ofxylanase and B-xylosidase was produced from mixed bacterial culturepreviously isolated from an aerobic digester. The batch hydrolysisaimed at the study of optimal parameters such as the amount ofsubstrate and enzyme and problems related to the decrease ofsaccharification rate. The continuous hydrolysis in membranereactor was to increase the efficiency of enzymatic hydrolysis ofcorn hull and to recycle the enzyme use.Results from batch process revealed that the optimalconcentration of corn hull was 3% (w/v). Increase the amount ofcorn hull rather than 3% decreased the percent of saccharification.The maximum saccharification was 55"b within 31 hours.Saccharification was not changed. at higher enzyme concentration butthe reaction was faster.Xylose was the most pentose sugars from the hydrolysis ofcorn hull. The amount of xylose in total reducing sugars dependedupon the availability of B-xylosidase in mixed enzyme preparation.When xylanase was not limited, 76% of reducing sugars was pentose.The enzyme required for the hydrolysis of 3% corn hull was 60unit/ml xylanase and 0.6 unit/ml B-xylosidase.The enzyme activity was still maintained after 48 hours ofexperiment. Therefore the ability of enzyme to degrade thes&&rate was limited due to the availability of hydrolyzablesubstrate and product inhibition. The use of membrane reactor withPIlO increased the efficiency of saccharification as high as 36% in33 hours. The enzyme was continuously used without any decrease inpercent saccharification at least for 20 hours.This research presents the study of enzymatic hydrolysis ofhemicellulose of corn hull to xylose. The enzyme composed ofxylanase and B-xylosidase was produced from mixed bacterial culturepreviously isolated from an aerobic digester. The batch hydrolysisaimed at the study of optimal parameters such as the amount ofsubstrate and enzyme and problems related to the decrease ofsaccharification rate. The continuous hydrolysis in membranereactor was to increase the efficiency of enzymatic hydrolysis ofcorn hull and to recycle the enzyme use.Results from batch process revealed that the optimalconcentration of corn hull was 3% (w/v). Increase the amount ofcorn hull rather than 3% decreased the percent of saccharification.The maximum saccharification was 55"b within 31 hours.Saccharification was not changed. at higher enzyme concentration butthe reaction was faster.Xylose was the most pentose sugars from the hydrolysis ofcorn hull. The amount of xylose in total reducing sugars dependedupon the availability of B-xylosidase in mixed enzyme preparation.When xylanase was not limited, 76% of reducing sugars was pentose.The enzyme required for the hydrolysis of 3% corn hull was 60unit/ml xylanase and 0.6 unit/ml B-xylosidase.The enzyme activity was still maintained after 48 hours ofexperiment. Therefore the ability of enzyme to degrade thes&&rate was limited due to the availability of hydrolyzablesubstrate and product inhibition. The use of membrane reactor withPIlO increased the efficiency of saccharification as high as 36% in33 hours. The enzyme was continuously used without any decrease inpercent saccharification at least for 20 hours.

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: รศ.ดร. รัตนา จิระรัตนานนท์

Role: ประธานกรรมการ

Name: รศ.ดร. มรกต ตันติเจริญ

Role: กรรมการ

Name: กนก รัตนกนกชัย

Role: กรรมการ

Name: ดร. ดุษฏี อุตภาพ

Role: กรรมการ

Created: 2533

Issued: 2005-04-02

Modified: 2553-07-10

วิทยานิพนธ์/Thesis

ISBN: 9748154572

CallNumber: BIT133

tha

DegreeName: วิทยาศาสตรมหาบัณฑิต

Level: ปริญญาโท

Descipline: เทคโนโลยีชีวภาพ

Grantor: มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

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3	BIT133ab.smi	2.01 KB	41	2023-12-26 13:37:54

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