แจ้งเอกสารไม่ครบถ้วน, ไม่ตรงกับชื่อเรื่อง หรือมีข้อผิดพลาดเกี่ยวกับเอกสาร ติดต่อที่นี่ ==>
หากไม่มีอีเมลผู้รับให้กรอก thailis-noc@uni.net.th
หากไม่มีอีเมลผู้รับให้กรอก thailis-noc@uni.net.th
Chatsri Deachapunya. Parameters affecting bile acid and bile calcium secretion. Master's Degree(Physiology). Mahidol University. Mahidol University Library and Knowledge Center. : Mahidol University, 1987.
Parameters affecting bile acid and bile calcium secretion
ปัจจัยที่มีผลต่อการหลั่งน้ำดีและแคลเซียมในน้ำดี
Name: Chatsri Deachapunya
MeSH:
Bile Acids and Salts
MeSH:
Hormones
MeSH:
Theophylline
Abstract:
Experiments were designed to investigate (i) influence of plasma Ca concentration on bile secretion; (ii) the role of Ca regulating hormones on bile Ca secretion; (iii) association of bile acid and bile Ca secretion; and (iv) possible route (s) of bile Ca secretion. The investigations were performed in fasted 170-200 g female Wistar rats which were groups into 8 groups as follows: normocalcemic, Ca gluconate-induced hypercalcemic and thyroparathyroidectomy and EGTA-induced hypocalcemic groups; each group was devided into the normal saline (normal bile flow) and sodium taurocholate-treated groups (choleresis). It was found that changes in plasma Ca concentration had no effect either on bile flow or bile acid concentration with an exception of the hypocalcemic groups which had a lower bile acid concentration when compared with the values of normocalcemic group. In contrast, the bile Ca concentration exhibited a linear correlation with the plasma Ca concentration with a ratio of 1, suggesting a passive transport of Ca from blood into bile. To test whether bile Ca secretion is controlled by Ca regulating hormones, experiments were done in thyroparathyroidectomized (TPTX) rats which received Ca gluconate solution to maintain normocalcemia (Ca clamp). Results showed that bile Ca concentration increases after Ca gluconate infusion, indicating that bile Ca secretion does not require the presence of Ca regulating hormones but is a direct function of the plasma Ca concentration. However, since bile Ca and bile acid concentrations were slightly but significantly lower than the values of normocalcemic control, the Ca regulating hormones may have some influences over the secretion of bile acid and Ca. The mechanism, however, is unknown. Plotting of bile Ca and bile acid secretion demonstrated a positive correlation in normocalcemic and hypercalcemic groups. However, the slope representing bile Ca secreted per unit change of bile acid significantly increased in the hypercalcemic groups and showed tendency to decrease in the bile acid-treated groups, when compared to the corresponding control, suggesting that bile acid and Ca secretions were not tightly coupled. Nevertheless, when the relationship of bile Ca secretion and bile flow was plotted, at the same bile flow rate, bile Ca secretion was slightly higher in the bile acid-treated group when compared to the normal saline control, indicating that bile acid has an additional effect on bile Ca secretion independent of its effect on bile flow. To investigate possible route(s) of bile Ca secretion, an intravenously infused (45)Ca used as a marker of Ca secreted from the extracellular fluid(ECF). Assumming that appearance of (45)Ca in bile within 3 min is indicative of paracellular transport and by calculating the ratio of Ca secreted from ECF and Ca secreted from cells, it was shown for the first time that approximately 80% of bile Ca was secreted from cells while 20% was secreted directly from the ECF, presumably via the paracellular pathway. Moreover, during the stimulation of bile acid independent bile flow (BAIF) by theophylline, although both portions of bile Ca were increased, the ratio was altered, i.e., the Ca secreted from ECF was increased to 25% while the Ca secreted from cells was decreased to 75%.
Mahidol University. Mahidol University Library and Knowledge Center
Address:
NAKHONPATHOM
Email:
liwww@mahidol.ac.th
Name: Nateetip Krishnamra
Role:
Thesis Advisors
Created:
1987
Modified:
2018-03-10
Issued:
2018-03-10
วิทยานิพนธ์/Thesis
application/pdf
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Physiology
Grantor: Mahidol University
ใช้เวลา
-0.589359 วินาที
Chatsri Deachapunya
| Title | Contributor | Type |
|---|---|---|
| Parameters affecting bile acid and bile calcium secretion
มหาวิทยาลัยมหิดล Chatsri Deachapunya | Nateetip Krishnamra | วิทยานิพนธ์/Thesis |
Nateetip Krishnamra