Abstract:
Samples from soil in the sisal plantation, dried sisal leaves and residues were collected and screened for microorganisms that produce enzyme cellulose. Twenty six isolates of bacteria and 23 isolates of fungi were obtained. Leave juice and waste water from rope manufacturing revealed 13 bacterial isolates and 14 isolates with activities for alcoholic fermentation. The cellulose producing microorganism are divided into two groups. The first group belongs to fungi with one important isolate identified as Botryotrichum sp. having cellulose activity of 0.74 Unit/ml. at 37ºC. The second group belongs to bacteria with one sample identified as Bacillus sp. having the cellulose activity of 0.033 Unit/ml. at 37ºC. The ethanol-producing microorganisms were also divided into two groups, with one being bacteria having an isolate identified as Candida sp., having capability to produce 80.57 mg./ml. of ethanol at 37ºC when Candida sp. and the enzyme cellulose from Botryotrichum sp. were used together in the simultaneous saccharification and fermentation (SSF) process, the ethanol yield was 5.79 mg./ml within 7 days