Somatic embryogenesis of (Saccharum officinarum var Hawaii) and isozyme comparison of regenerated plant by isoelectricfocusing

การเกิดคัพภะจากาโซมาติคเซลของอ้อย (Saccharum officinarum var. Hawaii) และการเปรียบเทียบไอโซไซม์ของพืชที่ได้จากการเพาะเลี้ยงโดยวิธีไอโซอีเลคทริคโฟกัสซิ่ง

Name: Laksana Kantama

LCSH: Isoelectirc focusing

LCSH: Isoenzymes

LCSH: Plants -- Embryology

LCSH: Somatic cells

LCSH: Sugarcane

Abstract: Callus induction was established from the young leaves of sugarcane (Saccharum officinarum var. Hawaii) on modified Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-D and 10% coconut water. The medium supplemented with 3 mg/l 2,4-D was the optimum on callus induction for the explants derived from 1.5-4 cm above the youngest node. While the medium supplemented with 3 mg/l 2,4-D and 10% coconut water produced green pin headed structures on the explant derived from 5-6 cm above the node. The calli were proliferated and maintained on modified MS medium supplemented with 0.5 mg/l 2,4-D. Embryogenic induction was achieved on modified MS medium with or without 0.5 mg/l 2,4-D and 10% coconut water. The embryogenic calli were transferred to modified MS medium containing 1.0% or 1.5% saccharosee for embrygenic development. In order to promote shoot elongation and pre-embryogenic callus recycling, the embryoid cluster was transferred to modified MS medium supplemented with 3 mg/l 2,4-D and 10% coconut water. Then the plantlets were transferred to modified MS medium supplemented with 1 mg/l IAA for for root development. The plantlets were transferred to pots with vermiculite and watered with complete fertilized for a month before establishing in soil. The regenerated plants could be derived in 3 groups by morphological comparisons. From isozyme isoelectricfocusing, there are 2 samples of regenerated plants showed two bands missing in esterase zymogram as compared to the donors.

Mahidol University. Mahidol University Library and Knowledge Center

Address: NAKHONPATHOM

Email: liwww@mahidol.ac.th

Name: Sarinee Chaicharean

Role: Thesis Advisors

Name: Vithoon Viyanant

Role: Thesis Advisors

Name: Thawat Tinnangwattana

Role: Thesis Advisors

Created: 1991

Modified: 2018-02-23

Issued: 2018-02-23

วิทยานิพนธ์/Thesis

application/pdf

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Environmental Biology

Grantor: Mahidol University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	10275617-eng.pdf	316.25 KB
2	10275617-tha.pdf	308.69 KB
3	10275617.pdf	24.69 MB

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