Roles of sericin protein from silkworm cocoons, bombyx mori, on human embryonic stem cells and wharton's jelly mesenchymal stem cells

บทบาทของโปรตีนซิริซินจากรังหนอนไหม (Bombyx mori) ต่อเซลล์ต้นกำเนิดตัวอ่อนและเซลล์ต้นกำเนิดวาร์ตันเจลลีมี-เซนไคม์ของมนุษย์

Name: Kewalin Inthanon

ThaSH: sericin

Classification :.DDC: 571.6

ThaSH: Cytology

Abstract: This study revealed, for the first time, the effects of sericin (SC) on two types of human stem cells, embryonic stem cells (hESC), H9 and Wharton’s jelly mesenchymal stem cells (hWJMSC), BCP-K1. The experiments were set up depended on the sensitivity of the cells and purposes of further applications. On the purpose of applying SC as a serum and/or growth factor replacement in H9 culture system, dissolved SC solution was prepared. Autoclaved SC at concentration 0.05% (w/v), as an effective concentration, enhanced cell attachment, viability and proliferation of H9 single cell. This concentration also increased numbers of survival cells but decreased injured and death cells in H9 colony. Initial attachment was activated by SC leading to increase the attachment protein (E-cadherin) and then enhance cell proliferation and the cell cycle protein (Ki-67) afterward. Neuroectodermal differentiation was detected at day 5 in SC culture. At day 15, pluripotency antigen makers and gene expressions were completely disappeared but with the highest expression of neuroectodermal genes. The neuroectodermal differentiation was initiated by SC on the proteins involved in ERK1/2 and CREB signaling pathway. The application of SC into stem cell culture system should be aware of timing for cellular differentiation. Induction of neuronal differentiation on hESC by SC would offer possible advantages to neuroregenerative medicine and cellular therapies. The aspect of using SC as a biomaterial for cellular therapies was considered. A novel electrospinning biodegradable co-polymer membrane (PLCL-SC) was fabricated from a synthetic polymer, poly (L-lactic-co-ε -caprolactone) (PLCL), blended with SC. BCP-K1 was chosen as a candidate cells with the ability to differentiate into several cell types. Membranes with concentrations of 2.5 or 5% (w/v) SC exhibited qualified texture characteristics with a non-cytotoxic release profile. Physicochemical properties of the membranes were evaluated. The hydrophilic properties of the membranes were 35-40% higher than those of a standard PLCL and commercial polystyrene (PS). The improved characteristics of the membranes were due to an addition of new functional amide groups; C=O, N-H and C-N, onto their surfaces. Degradation of the membranes was controllable, depending on the content proportion of SC. Results of thermogram indicated the superior stability and crystallinity of the membranes in comparison to the PLCL. These membranes enhanced BCP-K1 adhesion by up-regulating focal adhesion kinase (FAK) expression and promoted proliferation by increasing cyclin A expression. On the membrane, BCP-K1 selectively differentiated into neuronal precursors. PLCL2.5SC membrane was more biocompatible and less cytotoxic to BCP-K1 than the PLCL-5SC. These data suggest that PLCL-SC electrospun membrane represents a useful tool for tissue-engineering and medical applications.

Chiang Mai University Library

Address: CHIANG MAI

Email: cmulibref@cmu.ac.th

Name: Niwooti Whangchai

Role: Chairman

Name: Weerah Wongkham

Role: Member

Name: Vorathep Khaniyao

Role: Member

Name: Winita Punyodom

Role: Member

Name: Korakot Nganvongpanit

Role: Member

Created: 2015

Modified: 2017-09-17

Issued: 2017-09-17

วิทยานิพนธ์/Thesis

application/pdf

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Biology

Grantor: Chiang Mai University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	biol60715kit_full.pdf	6.35 MB	4	2019-06-10 12:08:40

ใช้เวลา

-0.960178 วินาที