Characterization of jatropha curcas l. latex

Name: Lucsame Gruneck

Organization : Mae Fah Luang University

keyword: Jatropha curcas

LCSH: Jatropha -- Biotechnology

Classification :.LCCS: QK495.E9

Abstract: Latex proteins have been previously characterized in various plants including their indispensable roles. However, latex chemical and protein profiles are not well defined in Jatropha, neither of toxic nor non-toxic varieties. Fourier Transform Infrared (FTIR) was used for analysis of functional groups in latex. The results from FTIR analysis of latex extracted from toxic and non-toxic Jatropha showed that the pattern of the infrared (IR) spectra of toxic was similar to non-toxic, and the functional groups of those two varieties were nearly identical to cis-1,4-polyisoprene reported in Hevea brasiliensis. The difference of peak intensity also reflected the concentration of compositions in latex of the two varieties. We further compared the protein profiles between toxic and non-toxic latex by using a proteomic approach (one-dimensional gel electrophoresis in conjunction with LC-MS/MS). Proteomic analysis of Jatropha latex revealed 421 proteins, of which 368 were shared between the two varieties. Functional classification showed that the most abundant proteins in both toxic and non-toxic Jatropha latex were related to cellular and metabolic processes, while some were associated with immune system processes. Furthermore, some unique proteins were classified as defense-related highlighting the special role of latex in the defense mechanism of Jatropha. Finally, we identified proteins belonging to the phenylpropanoid, carotenoid and flavonoid biosynthesis pathways. Furthermore, sequencing of Jcr4S01440.10 gene has confirmed the amplified product that matched to Jatropha curcas TMV resistance protein N-like. Quantitative PCR (qPCR) analysis of JcrLRR440 expression in response to mechanical wounding demonstrated that JcrLRR440 gene was down-regulated at all time points compared to the control treatment. Low level of gene expression in Jatropha leaves might be affected by some factor such as plant hormones during the post-wounding period. The results also suggested that time points later than 12 h should be observed. To the extent of our knowledge, this study provides the first comprehensive proteomic data for Jatropha latex and revealed the molecular function of LRR-encoding gene which might be useful for future research on genetic improvement of Jatropha for desirable traits.

Mae Fah Luang University. The Learning Resources and Education Media Center

Address: Chiang Rai

Email: library@mfu.ac.th

Name: Siam Popluechai

Role: Advisor

Name: Teerawit Waratrujiwong

Role: Co-Advisor

Created: 2016

Issued: 2560

Modified: 2020-04-29

Issued: 2017-08-16

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Thesis QK495.E9 L942c 2016

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biotechnology

Grantor: Mae Fah Luang University

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