Abstract:
Mycelial growth of Lentinulaedodes MU2 and Pleurotusostreatus NANGROM 1 were established in various natural liquid dextrose media prepared from produces including Potato (PD) , Corn (CoD), Soybean (SD), Cassava (CaD), white Raddish (WRD) , Papaya (PPD) , and Banana (BD) , in comparison to a synthetic medium (SM). Both mushrooms appeared to favor PD, as determined from the measurement of mycelial dry weight. L. edodes gave the mycelial mass of 0.817 g per 100 ml within 60 days and p. ostreatus0.972 g per 100 ml within 40 days. The supplementation of PD with coconut juice (PDC) was found to be superior for L. edodes , giving the mycelial mass of 1.040 g per 100 ml within 25 days, while L. ostreatusprefered the yeast extract (PDY), giving the mycelial mass of 1.131 g per 100 ml within 25 days. The optimum pH for L. edodes in PDC was at 5.0, giving the mycelial mass of 1.136 g per 100 ml within 40 days. P. ostreatus showed the optimum pH of 6.0 in PDY and yielded the mycelial mass of 1.464 g per 100 ml within 25 days. The optimum temperatures were at 25 °c for both mushrooms. L. edodes save the mycelial mass of 1.147 g per 100 ml within 40 days and P. ostreatus yielded 1.489 g per 100 ml within 25 days. Semistationary culture conditions were found to be more suitable than stationary culture condition,edodes gave the mycelial mass1.2 30 g per 100 ml within 40 days. P. ostreatus gave 1.534 g per 100 ml within 25 days.The scaleup of production to 51-fermentors suggested that aeration is essential. The mycelial dry weight of L.oedodes in PDC at pH 5.0 25 °c was 72.074 g per 5 1 (1.441 g per 100 ml) within 40 days P. ostreatus in PDY at pH 6.0 25 °c was 83.998 g per 5 1 (1.679 g per 100 ml) within 20 days.Both mushrooms produced spherical pellets under aerated contitions, while the mat formations were observed only when the fermentor was not aerated.