Abstract:
The simultaneous hydrolysis and fermentation (SHF) process has been used for the production of ethanol from lignocellulosic using one bioreactor with continuous reactions starting from the saccharification of cellulose to glucose followed by a rapid conversion of glucose by yeast to ethanol. Optimum conditions of the SHF process using Acrophialphora sp. Cellulose, Candida brassicae and Agave sisalana fiber were investigated. The condition of having 25 folds enzyme, 3 x 1010 cells/ml yeast incolum PH 5.0 at 45°C gave the maximum ethanol yields of 0.9125% (0.3042 g/g substrate) Addition of 0.05% casein peptone or 0.075% soy peptone as supplements were beneficial giving ethanol yields of 1.267% (0.422 g/g or 1.76 folds increased) from casein peptone and 0.9301% (0.310 g/g or 1.29 folds increased) from soy peptone respectively. Study of the co-cutured fermentation process using Acrophialphora sp. and Candida brassicae. Revealed that the use of 6 day-old fungal culture, 3 x 1010 yeast cells/ml, at 45°C gave ethanol yield of 0.7822% Z0.2607 g/g).