Effect of UV-C irradiation on postharvest quality of 'PHULAE' pineapple

Name: Sari, Lupita Kemala

Organization : Mae Fah Luang University

keyword: Postharvest quality

LCSH: Fruit -- Quality

Classification :.LCCS: SB360

LCSH: Fruit -- Postharvest technology

Abstract: The effect of UV-C irradiation on postharvest quality of pineapple cv. ‘Phulae’ was investigated. The pineapples were divided into 4 treatments: 10-min UV-C irradiation (13.2 kJ/m2), 20-min UV-C irradiation (26.4 kJ/m2), 30-min UV-C irradiation (39.6 kJ/m2) and no UV-C irradiation as a control. Quality properties including disease incidence, internal browning, color, total soluble solid (TSS), titratable acidity (TA), vitamin C content, total phenolic compound, total flavonoid content, antioxidant activity, peroxidase (POD) activity, and phenylalanine-ammonialyase (PAL) activity were measured every 7 days up to 28 days after irradiation. The results showed that UV-C irradiation significantly reduced (P < 0.05) disease incidence and internal browning in pineapple during storage at 10oC for 28 days. No significant difference among treatments were found in color, TSS and TA. The UV-C irradiation significantly increased (P < 0.05) total phenolic compound, total flavonoid and antioxidant capacity in peel. The POD and PAL activities remained higher in UV-C at dose of 39.6 kJ/m2 than those in other treatments. The determination of phenolic acid compound in pineapple was conducted both in pulp and peel samples treated with UV-C at dose of 39.6 kJ/m2 and untreated control by using HPLC and LC-MS. In both UV-C treated and untreated peel, 4 compounds were isolated and were identified as chlorogenic acid, p-coumaroylisocitrate (or p-coumaroylquinic acid), dicaffeoylglycerol and p-coumaroyl-caffeoylglycerol. Chlorogenic acid, p-coumaroylisocitrate (or p-coumaroylquinic acid) and sinapoylhexoside were identified in pulp, both treated and untreated with UV-C. Pineapple peel and pulp irradiated with UV-C, had higher amount of phenolic acid compound compared to the untreated control. The finding in this study suggests that UV-C irradiation could induce synthesis of phenolic compound and could be an alternative postharvest disinfestation treatment in ‘Phulae’ pineapple. The pineapple peel was used for further analysis in antimicrobial assays for controlling anthracnose disease caused by Colletotrichum gloeosporioides. The result showed that irradiated peel could inhibit the mycelial growth for 56.94% in comparison to unirradiated peel (52.36%) and distilled water (0%) at day 6 of incubation. The extract of irradiated peel also showed better result in suppressing spore germination in which the germination rate was 11.32%, where there were 17.33% and 45.70% in unirradiated peel extract and distilled water, respectively. The result suggested that irradited peel of pineapple could be effectively used as natural extract for controlling the growth of C. gloeosporioides in vitro.

Mae Fah Luang University. The Learning Resources and Education Media Center

Address: Chiang Rai

Email: library@mfu.ac.th

Name: Matchima Naradisorn

Role: Advisor

Name: Sutthiwal Setha

Role: Co-Advisor

Created: 2016

Issued: 2017

Modified: 2020-04-27

Issued: 2017-04-10

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Thesis SB360 S245e 2016

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Technology Management of Agricultural Produces

Grantor: Mae Fah Luang University

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