Abstract:
This study was carried out to determine effects of diet to reproductive performance of pond-reared male Penaeus monodon compared with pond- reared male before get the diet and the wild male prawn. Spermatophore weight, the percentages of normal sperms and live sperm, were used as sperm quality index. Composition of high unsaturated fatty acids such as AA (20: 4n-6), EPA (20: Sn-3) and DHA (22: 6n-3) in reproductive, muscle and hepatopancrease of males in each category also were determined. The results showed that pond-reared male prawn can be used as replacement of wild males broodstock because sperm quality of them were similar. One month in captivity did not affect sperm quality. Control (Live feed) or combined diet provided better reproductive performance in pond-reared males. The pelleted feeds, only the experimental diet and commercial maturation diet could not promote reproductive performance of pond-reared males. These may be lack of balance in high unsaturated fatty acids in the feeds. Compositions of high unsaturated fatty acids in reproductive organ of males, hepatopancreas and muscle, especially AA (20: 4n-6) DHA (22: 6n-3) and EPA (20: 5n-3) were similar to the diets, indicating that HUFA accumulation in the tissues was influenced by the diets.