Abstract:
The aim of this study was to develop facial loose powder containing mangosteen peel extract and to study its antimicrobial activities. The mangosteen extract possessed antibacterial activity against Staphylococcus aureus and Propionibacterium acnes by broth dilution method with the Minimal Inhibitory Concentration (MIC) of 2 ug/ml. The antimicrobial activity was also tested by Disc agar diffusion method and the results showed that it was able to inhibit Escherichia coli, Pseudomonas aeruginosa and Candida albican with diameters of 1.05, 1.00 and 1.10 cm, respectively. The standard loose powder was then developed to have fine grain, smooth and stick well on the skin. The loose powder containing mangosteen peel extract at different concentration was prepared according to the MIC results and its cosmetically acceptable properties. Extracts at of 2, 4, 6, 8 and 10% were added and it showed increase in yellow colour as the concentration of the extract is higher and the product with 6% extract showed the most satisfaction by 10 volunteers. Additionally, the total plate count of the products was investigated and found no contamination of any bacteria, yeast and fungus as per cosmetics rules and regulations. Moreover, the antimicrobial activity of the product was also tested by Disc agar diffusion method in vitro and the results showed that the product with mangosteen peel extract was more active than standard loose powder to inhibit Staphylococcus aureus and Propionibacterium acnes. Skin irritation test was conducted with 10 volunteers and found no irritation on the skin. Sensory test
was also conducted and it showed that all volunteers were very happy with glowing effect and satisfy with product overall properties. Finally, swab test was performed on 5 volunteers after applying the product for 30 minutes and the results showed that the powder with extract exhibited better ability to lower the amount of microbial on the skin in 60% of volunteers