The Effects of Natural Products Against Lead (Pb) Induced Neurotoxicity

Name: Moe PwintPhyu

keyword: Neurotoxicology Research

Classification :.LCCS: RC347.5 M693 2014

; Neurotoxic agents Research

; Lead Environmental aspects

Abstract: Lead (Pb) is a potent environmental toxic agent which leads to develop various hazardous effects on human health. Exposure to low level of Pb has been implicated in behavioral abnormalities and cognitive deficit. Lead Pb can generate free radical to inhibit enzyme acetylcholinesterase (AChE). Further, the existing synthetic drugs for Pb toxicity confer mild to severe side effect and some of drugs are known to exert their action via antioxidant activity. From this perspective, this study was designed to investigate the effects of total phenolic content, total antioxidant capacity and free radical scavenging activities in cell free system, the protective effects of Thunbergialaurifolia (Linn.) (TL), Xanthone derivative Garciniamangostana and Curcumin fromrhizomes of Curcuma longa against lead induced acetycholinesterase (AChE) dysfunction and cognitive impairment in mice. TL is a Thai medicinal plant known for its anti-mutagenic, anti-inflammatory and anti-pyretic properties.Garciniamangostana is an active antioxidant phytonutrient that neutralize free radicals and promote healthy cardiovascular, gastrointestinal, respiratory, immune and nervous systems. Curcumin from rhizomes of Curcuma longa, is a nutraceutical against numerous diseases in patients with various pro-inflammatory diseases including cancer, cardiovascular disease, arthritis, ulcerative colitis, gastric ulcer, atherosclerosis and diabetes. Based on these natural products which obtained from the plant sources are relatively less expensive, safe and possess good tolerability even in higher doses. ICR mice were exposed to lead acetate in drinking water (1%) with or without natural products orally daily administration (100 and 200 mg/kgBW) for 38 days. The results showed that Xanthonepossess higher phenolic content and antioxidant activitythan TL and Curcumin. The fifty percent of inhibitory concentration of TL, Xanthone and Curcumin on scavenging free radical activities (IC50) for hydroxyl radical, superoxide radical and hydrogen peroxide in cell free system were 0.48-1.47 ± 0.09-0.28, 1.86-2.23 ±0.06-0.1 and 1.1-2.2 ± 0.03-0.09 mg/mL, respectively. Exposure to Pb-induced AChE dysfunction and memory deficit in a dose dependent manner, indicated by in vitro and in vivo studies. However, treatment with natural products alleviated Pb-induced AChE dysfunction in blood and brains of mice. They also suppressed Pb-induced oxidative damage indicator bymalondialdehyde (MDA) and improved cognitive deficit and depression which indicated by Morris water maze and forced swimming test compared to Pb-exposure control group.Pretreatment with natural products can decrease the amount of oxidative stress led to inhibit AChE and ChAT dysfunction We could draw the conclusion that natural products could alleviatePb induced neurotoxicity, at least in part, by suppression oxidative damage, reversing acetylcholinesterase activity with a reduction learning deficit and memory loss and modulating Pb induced alteration in enzyme AChE and ChAT expression. Keywords: Thunbergialaurifolia (Linn.), Xanthone, Curcumin, Acetylcholinesterase (AChE), Choline acetyl transferase (ChAT), lead (Pb), oxidative stress

WALAILAK UNIVERSITY. CENTER FOR LIBRARY RESOURCES AND EDUCATIONAL MEDIA

Address: NAKON SI THAMMARAT

Email: clm@wu.ac.th

Name: Jitbanjong Tangpong

Role: Advisory Committe Chairman

Created: 2559

Modified: 2559-06-29

Issued: 2559-06-29

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: RC347.5 M693 2014

tha

DegreeName: Master of Science (Biomedical Sciences)

Level: Master's Degree

Descipline: Biomedical Sciences

Grantor: Walailak University

©copyrights มหาวิทยาลัยวลัยลักษณ์

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	Front page.pdf	75.9 KB	3	2018-06-08 09:05:43
2	Abstract.pdf	18.59 KB	2	2018-06-08 09:05:44
3	Chapter 1.pdf	48.51 KB	2	2018-06-08 09:05:45
4	Chapter 2.pdf	254.03 KB	2	2018-06-08 09:05:46
5	Chapter 3.pdf	93.52 KB	2	2018-06-08 09:05:47
6	Chapter 4.pdf	1.14 MB	2	2018-06-08 09:05:47
7	Chapter 5.pdf	1.17 MB	2	2018-06-08 09:05:48
8	Chapter 6.pdf	1.03 MB	2	2018-06-08 09:05:49
9	Chapter 7.pdf	192.71 KB	2	2018-06-08 09:05:51
10	Bibliography.pdf	95.45 KB	2	2018-06-08 09:05:52
11	CV.pdf	37.21 KB	2	2018-06-08 09:05:53

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