Optimizing gorwing conditions for the tropical mushrooms, clitopilus species and agaricus subrufescens

Name: Kritsana Jatuwong

Organization : Mae Fah Luang University

keyword: Mushrooms

LCSH: Mushrooms

Classification :.LCCS: QK617

Abstract: The aim of this study was to study taxonomy and phylogeny, optimal condition for mycelium growth, antibacterial, antioxidant, and enzyme activities of Clitopilus doimaesalongensis strain MFLUCC13-0806 and C. chalybescens strain MFLUCC13-0809 and to investigate the optimal conditions for the cultivation of Agaricus subrufescens hybrid strains in northern Thailand. Collection of Clitopilus were made in the rainy season during the period 2012-2013 in northern Thailand. They were studied in terms of taxonomy and phylogeny. Two collections were identified as Clitopilus chalybescens and another as a new species, C. doimaesalongensis. Clitopilus doimaesalongensis (MFLUCC13-0806) and C. chalybescens (MFLUCC13-0809) were determined for optimal growth conditions; type of media, temperature and pH. The optimal conditions for C. doimaesalongensis were cultivation on malt extract agar (MEA), supplemented with sucrose and tryptone, pH can range from 5-9 at a temperature range from 27-29˚C. Yeast extract agar (YEA), a pH of 5-8 and temperature range of 20-29˚C were optimal conditions for C. chalybescens and this mushroom can use fructose, sucrose, starch, malt extract, yeast extract, tryptone and beef extract as carbon and nitrogen sources. The antibacterial activity from culture mycelium extracts and broth cultured extracts of C. doimaesalongensis inhibited the growth of Gram positive bacteria; Bacillus subtilis and Staphylococcus aureus and Gram negative bacteria; Pseudomonas aeruginosa. This activity, however, was not observed on dried mycelial extracts. Clitopilus chalybescens could inhibit the growth of Gram positive bacteria; B. subtilis and S. aureus, but not Gram negative bacteria. Antioxidant assay indicated that the crude extracts had noticeable scavenging activity on 2,2-diphenyl-1-picryl-hydrazyle (DPPH) radical about 21.41% and 26.45% DPPH scavenging activity for C. chalybescens and 29.14% and 40.62% for C. doimaesalongensis at 40 and 50 mg/mL, respectively. Cellulase, xylanase and amylase activities were detected in C. doimaesalongensis. Agaricus subrufescens is an edible mushroom, one of the most important medicinal mushroom with high potential to treat many diseases. Several strains of A. subrufescens have been cultivated throughout the world, especially in Brazil. The Agaricus subrufescens hybrid strain used in this study has bred at INRA, France and successfully cultivated. The optimum conditions for the cultivation of A. subrufescens hybrid strains in northern Thailand were investigated in this study. Suitable temperatures and pH for mycelium growth were established on compost extract agar. The optimum temperature and pH for mycelial growth ranged from 25-30˚C and pH 7-9. The four hybrid strains could be grown and produced fruiting bodies on rice straw compost. Agaricus subrufescens hybrid of Thai and Brazilian CA 918-076  CA 454-4 (T2B) produced the highest yield (56.92 g/kg of compost) among the studied strain.

Mae Fah Luang University

Address: CHIANG RAI

Email: library@mfu.ac.th

Name: Pattana Kakumyan

Role: Advisor

Created: 2015

Modified: 2020-04-20

Issued: 2016-05-27

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Thesis QK617 K929o 2015

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biosciences

Grantor: Mae Fah Luang University

©copyrights Mae Fah Luang University

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