Taxonomy and phylogeny of phyllosticta

Name: Saowanee Wikee

Organization : Mae Fah Luang University

keyword: Taxonomy and phylogeny

Classification :.LCCS: Phyllosticta

; phyllosticta

Classification :.;: Taxonomy

Classification :.;: Plant pathogens

Abstract: Phyllosticta is one of the major plant pathogenic genera, whose species have a worldwide distribution. Phyllosticta species are responsible for numerous diseases including leaf spots and black spots on fruits. Several species have also been reported as saprobes and/or as endophytes of many host plants. Species recognition in Phyllosticta has historically been based on morphology, culture characters and host association. Although there have been several taxonomic revisions of this genus, there is still considerable confusion in the classification and identification of Phyllosticta and Guignardia species. This thesis provides additional knowledge on the taxonomy and relationships of Phyllosticta species based on morphological and phylogenetic data. The diversity of the genus Phyllosticta in Thailand was investigated in this study. Plant tissues (i.e., leaves and fruits) were collected and used to isolate the Phyllosticta species. Their host and fungal mode of life were also recorded. Of 43 Phyllosticta isolates, 39 isolates were identified as P. capitalensis; other species were also present including 2 isolates of P. cordylinophila, 1 isolate of P. citrimaxima sp. nov. and 1 isolate of P. mangiferaceae sp. nov. I then focused on P. capitalensis taxonomy due to its common occurrence (based on this study). The morphology of the species was observed and recorded; P. capitalensis CPC20252 was fully described as a representative for this species in this study. The phylogeny was further explored using multigene analysis (ITS, ACT and TEF). Apart from the data of Thai P. capitalensis, sequences of other P. capitalensis strains from the CBS were included. Based on the phylogenetic tree, the data revealed that P. capitalensis was monophyletic exhibiting a broad host range and worldwide distribution. In addition, my study revealed 15 new species (i.e., Phyllosticta citrimaxima sp. nov. and P. mangiferaceae sp. nov. were isolated from Thailand) based on analysis of multigene; ITS, LSU, ACT, TEF and GPDH sequence data for 160 Guignardia/Phyllosticta isolates. This finding also confirmed that species identification in Phyllosticta cannot be based only on morphological characters. Twelve species of Phyllosticta were also investigated and thus designated as epitypes (based on phylogenetic tree analysis). Finally, several type species of Guignardia and Phyllosticta were studied solely by morphological characters. Their detailed descriptions and illustrations are given with an expectation to use as a key reference for future study.

Mae Fah Luang University

Address: CHIANG RAI

Email: library@mfu.ac.th

Name: Kevin D. Hyde

Role: Advisor

Name: Ekachai Chukeatirote

Role: Co-Advisor

Created: 2013

Modified: 2020-03-30

Issued: 2015-06-24

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Thesis QK725 S239t 2013

eng

DegreeName: Dotor of Philosophy

Level: Doctoral Degree

Descipline: Biotechnology

Grantor: Mae Fah Luang University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	Abs.pdf	446.91 KB	4	2025-04-29 13:42:53
2	Appen.pdf	595.35 KB	3	2025-04-29 13:43:01
3	Ch1.pdf	1.19 MB	3	2025-04-29 13:43:07
4	Ch2.pdf	2.1 MB	3	2025-04-29 13:43:13
5	Ch3.pdf	1.35 MB	3	2025-04-29 13:43:19
6	Ch4.pdf	1.81 MB	3	2025-04-29 13:43:25
7	Ch5.pdf	603.41 KB	3	2025-04-29 13:43:33
8	Cov.pdf	450.16 KB	3	2025-04-29 13:43:39
9	Cont.pdf	662.33 KB	3	2025-04-29 13:43:44
10	Intro.pdf	536.93 KB	3	2025-04-29 13:43:49
11	Ref.pdf	906.11 KB	3	2025-04-29 13:43:55
12	Vitae.pdf	487.05 KB	3	2025-04-29 13:44:01

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