Pestalotiopsis : phylogeny and DNA barcoding

Name: Sajeewa Maharachchikumbura

Organization : Mae Fah Luang University

keyword: DNA barcoding

Classification :.LCCS: Biology -- Research

; Pestalotiopsis

Classification :.;: DNA -- Analysis

Classification :.;: Phylogeny

Abstract: The genus Pestalotiopsis has received much attention in recent years, not only because of its role as a plant pathogen, but also as a commonly isolated endophyte which has been shown to produce a wide range of chemically novel diverse metabolites. Pestalotiopsis consists of around 230 species, most of which were named according to their host associations. However, Phylogenetic analyses in combination with morphology and review of literatures have shown that the genus needs revision, and many of the traditional species may be spurious. This calls for critical re-examination of the genus, using both phenotypic studies and phylogenetic analyses of sequence data based on ex-type and ex-epitype cultures. In this study, we have studied the genus Pestalotiopsis and tested the use of various genes to resolve species boundaries. The 10 gene regions (ACT, β-tubulin, CAL, GPDH, GS, ITS, LSU, RPB 1, SSU and TEF1) were utilized to resolve cryptic Pestalotiopsis species, ITS, β –tubulin and TEF1 proved to be the better markers. The other gene regions were less successful in PCR amplification and/or resolving species. Out of tested locus, as a single gene TEF1 gave the highest species resolution/ PCR success and combination of ITS, β-tubulin and TEF1 gave the best resolution. Furthermore, we examined large number of Pestalotiopsis strains, which were isolated from various hosts and geographic origins. Phylogenetic relationships between these strains and other genera in the family Amphisphaeriaceae were resolved based on LSU sequence data. The phylogeny shows that Pestalotiopsis is a distinct clade in Amphisphaeriaceae and should be split in three groups; besides Pestalotiopsis, the two new genera Neopestalotiopsis and Pseudopestalotiopsis are proposed. Phenotypic analyses of conidial characters coupled with phylogenetic analyses of sequence data were used to clarify species boundaries in the three genera. Species of Pestalotiopsis were assigned to 19 sections, 40 new species were described, one species was epitypified and two ex-types were re-examined. Neopestalotiopsis protearum assign as the generic type of the newly proposed Neopestalotiopsis. In addition we described 19 new species, two species were epitypified, two ex-type were re-examine and six section names were introduced to the Neopestalotiopsis. Pseudopestalotiopsis theae placed as the generic type of Pseudopestalotiopsis; besides two new species were introduced and one species was epitypified.

Mae Fah Luang University

Address: CHIANG RAI

Email: library@mfu.ac.th

Name: Kevin David Hyde

Role: Advisor

Name: Ekachai Chukeatirote

Role: Co-Advisor

Created: 2013

Modified: 2020-03-30

Issued: 2015-04-16

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Thesis QU58.5 S158p 2013

eng

DegreeName: Dotor of Philosophy

Level: Doctoral Degree

Descipline: Biosciences

Grantor: Mae Fah Luang University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	Abs.pdf	511.72 KB	12	2025-04-29 09:57:27
2	Appen.pdf	12.6 MB	8	2025-04-29 09:57:35
3	Ch1.pdf	7 MB	8	2025-04-29 09:57:44
4	Ch2.pdf	3.45 MB	9	2025-04-29 09:57:52
5	Ch3.pdf	23.05 MB	9	2025-04-29 09:58:10
6	Ch4.pdf	16.52 MB	8	2025-04-29 09:58:20
7	Ch5.pdf	543.23 KB	12	2025-04-29 09:58:30
8	Cov.pdf	477.68 KB	8	2025-04-29 09:58:37
9	Cont.pdf	448.13 KB	10	2025-04-29 09:58:46
10	Intro.pdf	858.15 KB	9	2025-04-29 09:58:53
11	Intro-2.pdf	767.79 KB	8	2025-04-29 09:59:01
12	Ref.pdf	592.27 KB	7	2025-04-29 09:59:07
13	Vitae.pdf	434.95 KB	7	2025-04-29 09:59:14

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