Strains Characterization of Mycobacterium gordonae by Southern Hybridization

Name: Akhtarun Naher

Organization : Dhaka-Bangladesh. Mitford. Sir Salimullah Medical College. Microbiology Department

Name: Somsak Rienthong

Organization : Ministry of Public Health. Department of Communicable Diseases Control. Tuberculosis Division

Name: Dhanida Rienthong

Organization : Ministry of Public Health. Department of Communicable Diseases Control. Tuberculosis Division

Name: Prasit Palittapongarnpim

Organization : Mahidol University. Faculty of Science. Department of Microbiology

keyword: Strains characterization

; Hybridization

; Mycobacterium gordonae

Abstract: Mycobacterium gordonae is a scotochromogenic mycobacteria, previously considered as a saprophyte but is now recognized as a potential opportunistic pathogen. Patients are usually compromised and get infected by the bacteria from environmental sources. In order to determine the sources of infection, a method for differentiating strains of M. gordonae is needed. In this study differentiation of the bacteria was tested by Southern hybridization of BsEIl-digested chromosomal DNA using a polymorphic DNA fragment of M. tuberculosis H37Rv previously cloned in our laboratory as probe. 23 isolates of M. gordonaecould be differentiated into 13 types with the most common hybridization pattern being shared by 4 isolates. The method may be useful for tracing the transmission of M. gortionaefrom environmental sources to human.

Mahidol University. Mahidol University Library and Knowledge Center

Address: NAKHONPATHOM

Email: liwww@mahidol.ac.th

Created: 2013

Modified: 2013-03-08

Issued: 1998-01/06

บทความ/Article

application/pdf

Mahidol Journal. 5, 1 (Jan.-June 1998) 29-30

eng

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