Genetic diversity of Thai banana (Musa) Cultivars using AFLPs

ความหลากหลายทางพันธุกรรมของพันธุ์กล้วยไทยด้วยเทคนิค AFLPs.

Name: Sirapope Wongniam

LCSH: Banana

LCSH: Banana -- Genetics

LCSH: DNA fingerprinting of plants

Abstract: Bananas (Musa) are one of the most important tropical fruit crops in Thailand which believed to be one as the centers of origins. To assess genetic diversity and determine genetic relationships of edible bananas in Thailand, 135 accessions of Musa cultivars and species were collected from villages, and natural locations were investigated, with wild M. itinerans served as an outgroup taxon. The Amplified Fragment Length Polymorphism (AFLP) analyses of eight primer combinations revealed a total of 730 polymorphic bands and 136 unique marker bands on these Musa accessions. UPGMA clustering of numerical data from AFLP patterns showed two large groups that corresponded to genome designations of M. acuminata (A) and M. balbisiana (B), which were known ancestors of most edible cultivars. The AFLP results can be used to distinguish M. acuminata subspecies, and M. balbisiana. The AFLP data suggested that Thai bananas, i.e. AA, AAA and some AAB cultivars are closely related to M acuminata subsp. malaccensis. Two forms of this subspecies were found: the typical ‘malaccensis’ and ‘Kra Isthmus’ forms. The first was commonly found in southern Thailand and in a new location in the North, while the latter was found only in Ranong and nearby provinces. The ABB, BBA and BBB cultivars were closely related to wild M. balbisiana in Thailand and the others have probably been imported from somewhere else such as the Pacific Islands. Unique marker bands showed different characteristics among M. balbisiana and related cultivated bananas and the subspecies within M. acuminata. The analyses were useful to infer progenitors of these cultivars, pronounce wide genetic diversity of the bananas in Thailand, and encourage for more wild accessions from different geographic origins to be studied.

Mahidol University. Mahidol University Library and Knowledge Center

Address: NAKHONPATHOM

Email: liwww@mahidol.ac.th

Name: Sasivimon Swangpol

Role: Thesis Advisors

Created: 2008

Modified: 2553-09-17

Issued: 2010-07-14

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: TH S252g 2008

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Plant Science

Grantor: Mahidol University

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