Abstract:
Screening and isolation of thrautochytrids from fallen senescent leaves of 3 mangrove trees in May and September were carried out at Leam Pak Bia, Phetchaburi Province. It was found that the percentage of occurrence of thrautochytrids in May (96.11%, 286 Isolates) was higher than that in September (72.22%, 155 Isolates). The highest percentage of ocurrence of thrautochytrids in May was Schizochytrium limacinum (28.89%), followed by Schizochytrium mangrovei (27.22%), Schizochytrium sp. 1 (8.33), Thraustochytrid 1 (1.67%) and Schizochhytrium sp. 2 (0.56%), respectively. While in September, the percentage of occurrence of Schizochytrium limacinum was 59.44% followed by Schizochytrium mangrovei (32.22%), Schizochytrium sp. 2 (8.33), Ulkenia visurgensis (1.67%) and Schizochytrium sp. 1 (0.56%), respectively. The culture of thrautochytrids in GYP (6%: 1%: 1%) at 25 ํC, 200 rpm for 4 days was carried on for fatty acid profiles. The results showed that docosahexaenoic acid (DHA) was the highest fatty acid as 0.98-243.19 mg/g dry weight (0.55-49.47% of Total Fatty Acid), while ecosapentaenoic acid and arachidonic acid were 0.18-6.47 mg/g dry weight (0.08-5.65% of Total Fatty Acid) and 0.20-9.88 mg/g weight (0.10-14.23% of Total Fatty Acid), respectively.