The immune response of the asian sea bass (Lates calcarifer, Bloch) and its susceptibility to Vibrio vulnificus infection in relationto benzo (a) pyrene exposure

Name: Kunlawan Sorach

keyword: Lates calcarifer

Abstract: Asian sea bass (Lates calcarife, Bloch), is important to local businesses and coastal aquaculture. It can live in sea water, brackish water, and fresh water. River mouths and coastal areas of Thailand have been contaminated from pollutants from terrestrial drainage. In addition, there are many oil spill reports to the sea, especially in the year 1999, a leakage in an alarming level to the aquatic environment. This current study researched the alteration in health and resistance ability of infectious disease in benzo(a)pyrene treated Asian sea bass. The fish were injected with a single dose of benzo(a) pyrene, a chemical composition of PAHs, with different concentration levels of .01, 1.0, and 10.0 mg/Kg (fish body weight) of 5 fish per group. Six days after benzo(a)pyrene injection, the innate immune response from the fish had depreciated. Most importantly, with the highest level of benzo(a)pyrene (10.0 mg/Kg fish B.W.), the total white blood cell count in peripheral blood and kidney had decreased 2.5 and 2 times, respectively. The intracellular superoxide production of kidney cells had decreased, which was statistically different (p<=0.05) as compared to the control fish. Moreover, the ability of white blood cells to engulf yeast cells (Saccharomyces cerevisiae) or phagocytic activity had lessen 31.6% and 17.6% from the concentration level of 1.0, and 10.0 mg/Kg (fish B.W.), respectively, which was statically different (p<=0.01) as compared to the control group. Furthermore, from the research concerning the benzo(a)pyrene injected host's efficiency in clearing bacterial pathogen, Vibrio vulnificus, which caused Vibriosis, had been found that the ability to get rid of bacteria out of fish's body had declined according to the greater concentration levels of benzo(a)pyrene. The total white blood cell count of peripheral blood had decreased in relation to the clearance efficiency of V.vulnificus in 48 hours. The symptom of bacterial infection had severely increased through time, and the clearance efficiency had depreciated. In conclusion, the innate immunity and bacterial resistance ability suppressed in Asian sea bass was dependent on dose exposure to benzo(a)pyrene. Asian sea bass could be a sensitive model in testing immunotoxicity because of its immediate response to toxic substance.

Burapha University. Library.

Address: (Chonburi)

Email: buulibrary@buu.ac.th

Name: Praparsiri Barnette

Role: Principal advisor

Name: Pichan Sawangwong

Role: Member

Name: Suwanna Panutrakul

Role: Member

Created: 2006

Issued: 2549-06-28

Modified: 2549-11-28

วิทยานิพนธ์/Thesis

ISBN: 9745027502

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Environmental Science

Grantor: Burapha University

©copyrights Burapha University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	title.pdf	236.41 KB	6	2018-05-20 14:41:39
2	chapter1.pdf	163.49 KB	10	2020-10-05 15:41:11
3	chapter2.pdf	836.66 KB	13	2020-10-05 15:41:51
4	chapter3.pdf	250.87 KB	7	2018-05-20 14:41:41
5	chapter4.pdf	843.67 KB	4	2018-05-20 14:41:42
6	chapter5.pdf	517.39 KB	4	2018-05-20 14:41:43
7	bibliography.pdf	413.69 KB	4	2020-04-29 01:51:38
8	appendix.pdf	288.69 KB	4	2020-04-29 01:51:52

ใช้เวลา

0.041686 วินาที