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Kiattawee Chuwongkomon. Proteomic and Characterization of α-amylase Inhibitor from Mungbean (Vigna radiate). (). Kasetsart University. Office of the University Library. : , ม.ค.-ธ.ค. 2551.
Proteomic and Characterization of α-amylase Inhibitor from Mungbean (Vigna radiate)
Name: Anussorn Wisessing
Organization :
Kasetsart University. Faculty of Science. Department of Biochemistry
Name: Arunee Engkagul
Organization :
Kasetsart University. Faculty of Science. Department of Biochemistry
Name: Arunee Wongpiyasatid
Organization :
Kasetsart University. Faculty of Science. Department of Applied Radiation and Isotopes
Name: Kiattawee Chuwongkomon
Organization :
Kasetsart University. Faculty of Science. Department of Biochemistry
keyword:
mungbean seeds
Abstract:
Callosobruchus maculatus, the insect pest, causes damaging to stored mungbean seeds leading to economical losses. For this reason the use of α-amylase inhibitors to interfere the pest digestion process has been an interesting alternative as a biocontrolling agent. The studies of gamma irradiated mungbean mutant, M5-16, demonstrated that α-amylase inhibitor from the mutant line was more inhibitory effective on C. maculatus α-amylase than its wild type, KPS1. To investigate this mechanism, both proteomics and biochemical characterization were employed. The α-amylase inhibitor from mungbean seed, M5-16, was purified in a two-step procedure involving ammonium sulfate precipitation and gel filtration chromatography using a sephadex G-100 column. This α-amylase inhibitor existed as a monomer and had a molecular weight of 27 kDa. Moreover, It was very interesting that α-amylase inhibitor from the mungbean seed inhibited C. maculatus α-amylase but did not inhibit human salivary α-amylase. 2D gel electrophoresis suggested that the gamma-induced mutant line has decreased expression of α-amylase inhibitor and other proteins comparing with its wild type.
Kasetsart University. Office of the University Library
Address:
BANGKOK
Email:
tdckulib@ku.ac.th
Created:
ม.ค.-ธ.ค. 2551
Modified:
2009-10-15
Issued:
2009-10-15
บทความ/Article
application/pdf
วิทยาสารเกษตรศาสตร์ (สาขาวิทยาศาสตร์), 42(5) หน้า 245-250
eng
©copyrights Kasetsart University
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ใช้เวลา
0.040406 วินาที
Anussorn Wisessing
| Title | Contributor | Type |
|---|---|---|
| Purification and Characterization of C. maculatus α-amylase
มหาวิทยาลัยเกษตรศาสตร์ Anussorn Wisessing;Arunee Engkagul;Arunee Wongpiyasatid;Kiattawee Chuwongkomon | บทความ/Article | |
| Proteomic and Characterization of α-amylase Inhibitor from Mungbean (Vigna radiate)
มหาวิทยาลัยเกษตรศาสตร์ Anussorn Wisessing;Arunee Engkagul;Arunee Wongpiyasatid ;Kiattawee Chuwongkomon | บทความ/Article | |
| Purification and characterization of a-amylase inhibitor from standard variety and M5-16 line of mungbean (Vigna radiata)
มหาวิทยาลัยเกษตรศาสตร์ Anussorn Wisessing | Kiattawee Choowongkomon Arunee Engkagul Arunee Wongpiyasatid | วิทยานิพนธ์/Thesis |
Arunee Engkagul
| Title | Contributor | Type |
|---|---|---|
| Characterization and Activity of Digestive Enzymes in Different Sizes of Nile Tilapia (Oreochromis niloticus L.)
มหาวิทยาลัยเกษตรศาสตร์ Rungkan Klahan;Nontawith Areechon;Ruangvit Yoonpundh;Arunee Engkagul | บทความ/Article | |
| Changing in TSS, TA and Sugar Contents and Sucrose Synthase Activity in Ethephon-Treated Pattavia Pineapple Fruit
มหาวิทยาลัยเกษตรศาสตร์ Ngarmnij Chuenboonngarm;Niran Juntawong;Arunee Engkagul;Wallop Arirob;Surin Peyachoknakul | บทความ/Article | |
| Chitinase and Carbonic Anhydrase Activities during Molting Cycle of Mud Crab (Scylla serrata Forskal 1775)
มหาวิทยาลัยเกษตรศาสตร์ Jintana Salaenoi;Mingkwan Mingmuang;Arunee Engkagul;Prathak Tabthipwon;Amara Thongpan | บทความ/Article | |
| Gamma-Irradiation Induced Alterations in Mungbean α-amylase Inhibitory Activities : Effects on α-amylase and Development of Mungbean Weevil (Callosobruchus maculatus)
มหาวิทยาลัยเกษตรศาสตร์ Arunee Engkagul;Praparat Hormchan;Arunee Wongpiyasatid | บทความ/Article | |
| The Comparative Effects of Corn and Cassava Diets on Physiological Properties of Gastrointestinal Tract of Broilers
มหาวิทยาลัยเกษตรศาสตร์ Suwanna Promthong;Uthai Kanto;Chanin Tirawattanawanich;Soontaranee Tongyai;Supaporn Isariyodom;Kanchana Markvichitr;Arunee Engkagul | บทความ/Article | |
| Purification and Characterization of C. maculatus α-amylase
มหาวิทยาลัยเกษตรศาสตร์ Anussorn Wisessing;Arunee Engkagul;Arunee Wongpiyasatid;Kiattawee Chuwongkomon | บทความ/Article | |
| Proteomic and Characterization of α-amylase Inhibitor from Mungbean (Vigna radiate)
มหาวิทยาลัยเกษตรศาสตร์ Anussorn Wisessing;Arunee Engkagul;Arunee Wongpiyasatid ;Kiattawee Chuwongkomon | บทความ/Article | |
| Characterization of digestive enzymes from adult freshwater peartl mussel chamberlainia hainesiana (Lea, 1856)
มหาวิทยาลัยขอนแก่น Wilailuk Khrueanet;Uthaiwan Kovitvadhi;Arunee Engkagul;Satit Kovitvadhi;Krisna Rungruangsak-Torrissen | บทความ/Article |
Arunee Wongpiyasatid
| Title | Contributor | Type |
|---|---|---|
| Proteomic and Characterization of α-amylase Inhibitor from Mungbean (Vigna radiate)
มหาวิทยาลัยเกษตรศาสตร์ Anussorn Wisessing;Arunee Engkagul;Arunee Wongpiyasatid ;Kiattawee Chuwongkomon | บทความ/Article |
Kiattawee Chuwongkomon
| Title | Contributor | Type |
|---|---|---|
| Purification and Characterization of C. maculatus α-amylase
มหาวิทยาลัยเกษตรศาสตร์ Anussorn Wisessing;Arunee Engkagul;Arunee Wongpiyasatid;Kiattawee Chuwongkomon | บทความ/Article | |
| Proteomic and Characterization of α-amylase Inhibitor from Mungbean (Vigna radiate)
มหาวิทยาลัยเกษตรศาสตร์ Anussorn Wisessing;Arunee Engkagul;Arunee Wongpiyasatid ;Kiattawee Chuwongkomon | บทความ/Article |