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Somwong Tragulrung. Mycorrhizal Fungi from Spathoglottis plicata and the Use of these Fungi to Germinate Seeds of S. plicata in vitro. (). Kasetsart University. Office of the University Library. : , ม.ค-มี.ค. 2547.
Mycorrhizal Fungi from Spathoglottis plicata and the Use of these Fungi to Germinate Seeds of S. plicata in vitro
Name: Pornpimon Athipunyakom
Organization :
Department of Agriculture. Plant Protection Research and Development Office. Plant Pathology Group
Name: Leka Manoch
Organization :
Kasetsart University. Faculty of Agriculture. Department of Plant Pathology
Name: Chitrapan Piluek
Organization :
Kasetsart University. Faculty of Agriculture. Department of Horticulture
Name: Suparp Artjariyasripong
Organization :
TISTR-MIRCEN
Name: Somwong Tragulrung
Organization :
Kasetsart University, Kamphaeng Saen Campus. DNA Technology Laboratory
keyword:
mycorrhizal fungi
;
in vitro
Abstract:
Healthy roots from mature plants of terrestrial orchid, Spathoglottis plicata (Blume.) collected from Chiang Mai, Chanthaburi, Nakhon Ratchasima and Bangkok were used to isolate mycorrhizal fungi. A modification of Masuhara and Katsuyas method was employed to isolate these fungi from the peloton. Identification was based on morphological characteristics. Nuclei were stained with safranin O using Bandonis method revealing that all isolates were binucleate. Three genera and four species of mycorrhizal fungi were identified: Epulorhiza repens, Rhizoctonia globularis, Rhizoctonia sp. and Sabacina sp. (Epulorhiza sp. anamorph). In vitro, symbiotic seed germination and development on seeds of S. plicata were tested using Epulorhiza repens and Rhizoctonia globularis. It was found that 99.2% of seeds inoculated with Epulorhiza repens and 96.3% of seeds inoculated with Rhizoctonia globularis germinated within 21 days, whereas only 15% of seeds incubated in the absence of fungi germinated. About 8.1% of seeds inoculated with Epulorhiza repens and 1.7% of seeds inoculated with Rhizoctonia globularis initiated leaves after 60 days of incubation, whereas in the absence of fungi they did not develop into seedlings and mortality gradually occurred. The seedlings became more mature and ready to be planted after 127 days.
Kasetsart University. Office of the University Library
Address:
BANGKOK
Email:
tdckulib@ku.ac.th
Created:
ม.ค-มี.ค. 2547
Modified:
2009-10-08
Issued:
2009-10-08
บทความ/Article
application/pdf
วิทยาสารเกษตรศาสตร์ (สาขาวิทยาศาสตร์), 38(1) หน้า 83-93
eng
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.039615 วินาที
Pornpimon Athipunyakom
| Title | Contributor | Type |
|---|---|---|
| Mycorrhizal fungi of terrestrial orchids : isolation, identification and symbiotic germination
มหาวิทยาลัยเกษตรศาสตร์ Pornpimon Athipunyakom | Leka Manoch | วิทยานิพนธ์/Thesis |
| Mycorrhizal Fungi from Spathoglottis plicata and the Use of these Fungi to Germinate Seeds of S. plicata in vitro
มหาวิทยาลัยเกษตรศาสตร์ Pornpimon Athipunyakom;Leka Manoch;Chitrapan Piluek;Suparp Artjariyasripong;Somwong Tragulrung | บทความ/Article | |
| Isolation and Identification of Mycorrhizal Fungi from Eleven Terrestrial Orchids
มหาวิทยาลัยเกษตรศาสตร์ Pornpimon Athipunyakom;Leka Manoch;Chitrapan Piluek | บทความ/Article |
Leka Manoch
| Title | Contributor | Type |
|---|---|---|
| Diversity and Distribution of Hyphomycetes from Dung in Thailand
มหาวิทยาลัยเกษตรศาสตร์ Onuma Jeamjitt;Leka Manoch;Niphon Visarathanonth;Chiradej Chamswarng | บทความ/Article | |
| Mycorrhizal Fungi from Spathoglottis plicata and the Use of these Fungi to Germinate Seeds of S. plicata in vitro
มหาวิทยาลัยเกษตรศาสตร์ Pornpimon Athipunyakom;Leka Manoch;Chitrapan Piluek;Suparp Artjariyasripong;Somwong Tragulrung | บทความ/Article | |
| Thermotolerant and Thermoresistant Paecilomyces and its Teleomorphic States Isolated from Thai Forest and Mountain Soils
มหาวิทยาลัยเกษตรศาสตร์ Luangsa-ard, Janet Jennifer ;Leka Manoch;Hywel-Jones, Nigel ;Suparp Artjariyasripong;Samson, Robert A | บทความ/Article | |
| Isolation and Identification of Mycorrhizal Fungi from Eleven Terrestrial Orchids
มหาวิทยาลัยเกษตรศาสตร์ Pornpimon Athipunyakom;Leka Manoch;Chitrapan Piluek | บทความ/Article |
Chitrapan Piluek
| Title | Contributor | Type |
|---|---|---|
| Mycorrhizal Fungi from Spathoglottis plicata and the Use of these Fungi to Germinate Seeds of S. plicata in vitro
มหาวิทยาลัยเกษตรศาสตร์ Pornpimon Athipunyakom;Leka Manoch;Chitrapan Piluek;Suparp Artjariyasripong;Somwong Tragulrung | บทความ/Article | |
| Isolation and Identification of Mycorrhizal Fungi from Eleven Terrestrial Orchids
มหาวิทยาลัยเกษตรศาสตร์ Pornpimon Athipunyakom;Leka Manoch;Chitrapan Piluek | บทความ/Article |
Suparp Artjariyasripong
| Title | Contributor | Type |
|---|---|---|
| Mycorrhizal Fungi from Spathoglottis plicata and the Use of these Fungi to Germinate Seeds of S. plicata in vitro
มหาวิทยาลัยเกษตรศาสตร์ Pornpimon Athipunyakom;Leka Manoch;Chitrapan Piluek;Suparp Artjariyasripong;Somwong Tragulrung | บทความ/Article | |
| Thermotolerant and Thermoresistant Paecilomyces and its Teleomorphic States Isolated from Thai Forest and Mountain Soils
มหาวิทยาลัยเกษตรศาสตร์ Luangsa-ard, Janet Jennifer ;Leka Manoch;Hywel-Jones, Nigel ;Suparp Artjariyasripong;Samson, Robert A | บทความ/Article |
Somwong Tragulrung
| Title | Contributor | Type |
|---|---|---|
| Mycorrhizal Fungi from Spathoglottis plicata and the Use of these Fungi to Germinate Seeds of S. plicata in vitro
มหาวิทยาลัยเกษตรศาสตร์ Pornpimon Athipunyakom;Leka Manoch;Chitrapan Piluek;Suparp Artjariyasripong;Somwong Tragulrung | บทความ/Article |