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Sunanta Ratanapo. Antibody-Binding Motif of Mimetic Peptides to V. cholerae O139 Lipopolysaccharide. (). Kasetsart University. Office of the University Library. : , ม.ค.-มิ.ย. 2549.
Antibody-Binding Motif of Mimetic Peptides to V. cholerae O139 Lipopolysaccharide
Name: Aree Thattiyaphong
Organization :
Kasetsart University. Interdisciplinary Bioscience Program
Name: Amornrat Promboon
Organization :
Kasetsart University. Faculty of Science. Department of Biochemistry
Name: Chaivat Kittigul
Organization :
Kasetsart University. Faculty of Science. Department of Microbiology
Name: Krongkaew Supawat
Organization :
Ministry of Public Health. Department of Medical Sciences. National Institute of Health
Name: Pathom Sawanpunyalert
Organization :
Ministry of Public Health. Department of Medical Sciences. National Institute of Health
Name: Sunanta Ratanapo
Organization :
Kasetsart University. Faculty of Science. Department of Biochemistry
keyword:
Vibrio cholerae O139
Abstract:
This study explores deduced amino acid sequences of mimetic peptides of Vibrio cholerae O139 epitopes in order to design specific antigens for use in diagnostic method. Mimetic peptides expressed on E. coli flagella were selected from a FliTrx random peptide library via the interaction with purified monoclonal antibody to V. cholerae O139. Inserted nucleotides encoding bound peptides were determined by PCR. Peptides from clones giving positive results were confirmed by Western blot analysis. Sixty-two positive E. coli colonies were obtained and nucleotide-sequenced. Inserted nucleotides were translated into amino acids. Fifty-six patterns of deduced amino acid sequences were obtained without a consensus sequence. Most sequences of mimetic peptides have amino acid motif as RXXR with approximate molecular weight of 1,700 to 2,000. Arginine and glycine occupy the highest percentage of amino acid composition.
Kasetsart University. Office of the University Library
Address:
BANGKOK
Email:
tdckulib@ku.ac.th
Created:
ม.ค.-มิ.ย. 2549
Modified:
2009-09-22
Issued:
2009-09-22
บทความ/Article
application/pdf
วิทยาสารเกษตรศาสตร์ (สาขาวิทยาศาสตร์), 40(5) หน้า 90-95
eng
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.048448 วินาที
Aree Thattiyaphong
| Title | Contributor | Type |
|---|---|---|
| Pattern of English-Mixing Words in Thai Scientific Papers Published in the Bulletin of the Department of Medical Sciences
มหาวิทยาลัยรามคำแหง Aree Thattiyaphong | Wittaya Nathong Boonchan Thongprayoon | วิทยานิพนธ์/Thesis |
| Antibody-Binding Motif of Mimetic Peptides to V. cholerae O139 Lipopolysaccharide
มหาวิทยาลัยเกษตรศาสตร์ Aree Thattiyaphong;Amornrat Promboon;Chaivat Kittigul;Krongkaew Supawat;Pathom Sawanpunyalert;Sunanta Ratanapo | บทความ/Article |
Amornrat Promboon
| Title | Contributor | Type |
|---|---|---|
| Antibody-Binding Motif of Mimetic Peptides to V. cholerae O139 Lipopolysaccharide
มหาวิทยาลัยเกษตรศาสตร์ Aree Thattiyaphong;Amornrat Promboon;Chaivat Kittigul;Krongkaew Supawat;Pathom Sawanpunyalert;Sunanta Ratanapo | บทความ/Article | |
| Inhibitory Effects of Mulberry Leaf Lectins to Silkworm Proteases
มหาวิทยาลัยเกษตรศาสตร์ Sunanta Ratanapo;Yuwadee Thawalvichit;Amornrat Promboon | บทความ/Article |
Chaivat Kittigul
| Title | Contributor | Type |
|---|---|---|
| Antibody-Binding Motif of Mimetic Peptides to V. cholerae O139 Lipopolysaccharide
มหาวิทยาลัยเกษตรศาสตร์ Aree Thattiyaphong;Amornrat Promboon;Chaivat Kittigul;Krongkaew Supawat;Pathom Sawanpunyalert;Sunanta Ratanapo | บทความ/Article | |
| Studies on susceptibility and transovarial transmission of dengue virus type 2 in Aedes Aegyti mosquito strains collected in Thailand
มหาวิทยาลัยมหิดล Chaivat Kittigul | Pantipa Sinarachatanant | วิทยานิพนธ์/Thesis |
Krongkaew Supawat
| Title | Contributor | Type |
|---|---|---|
| Antibody-Binding Motif of Mimetic Peptides to V. cholerae O139 Lipopolysaccharide
มหาวิทยาลัยเกษตรศาสตร์ Aree Thattiyaphong;Amornrat Promboon;Chaivat Kittigul;Krongkaew Supawat;Pathom Sawanpunyalert;Sunanta Ratanapo | บทความ/Article |
Pathom Sawanpunyalert
| Title | Contributor | Type |
|---|---|---|
| Antibody-Binding Motif of Mimetic Peptides to V. cholerae O139 Lipopolysaccharide
มหาวิทยาลัยเกษตรศาสตร์ Aree Thattiyaphong;Amornrat Promboon;Chaivat Kittigul;Krongkaew Supawat;Pathom Sawanpunyalert;Sunanta Ratanapo | บทความ/Article |
Sunanta Ratanapo
| Title | Contributor | Type |
|---|---|---|
| Efficacy of Fermented Teas in Antibacterial Activity
มหาวิทยาลัยเกษตรศาสตร์ Sulak Talawat;Pimpan Ahantharik;Sajeena Laohawiwattanakul;Apinya Premsuk;Sunanta Ratanapo | บทความ/Article | |
| Antibody-Binding Motif of Mimetic Peptides to V. cholerae O139 Lipopolysaccharide
มหาวิทยาลัยเกษตรศาสตร์ Aree Thattiyaphong;Amornrat Promboon;Chaivat Kittigul;Krongkaew Supawat;Pathom Sawanpunyalert;Sunanta Ratanapo | บทความ/Article | |
| Inhibitory Effects of Mulberry Leaf Lectins to Silkworm Proteases
มหาวิทยาลัยเกษตรศาสตร์ Sunanta Ratanapo;Yuwadee Thawalvichit;Amornrat Promboon | บทความ/Article | |
| Partial Purification of Mulberry (Morus rotunbiloba) Peroxidase Using Aqueous Two-Phase Extraction Coupling with Ion-exchange and Gel-filtration Chromatography
มหาวิทยาลัยเกษตรศาสตร์ Supannapa Luanghiran;Poontariga Harinnasut;Amara Thongpan;Amonrat Proomboon;Sunanta Ratanapo | บทความ/Article | |
| Monodin, a new sialic acid-specific lectin from black tiger prawn (Penaeus monodon)
มหาวิทยาลัยมหิดล Sunanta Ratanapo | Montri Chulavatnatol Bhinyo Panijpan Stitaya Sirisinha | วิทยานิพนธ์/Thesis |