Abstract:
Three carbon sources: wastewater from canned fruit juice plant, biodiesel waste and sodium acetate, were used as a carbon source and electron donor for denitrification process. Denitrifying bacteria were taken from a wastewater treatment plant. Sodium acetate as a carbon source in stock culture. Denitrifying stock culture was maintained in the synthetic medium with anaerobic condition at room temperature. In batch experiment, microbial cultures were control at 2,000 mg MLVSS/l and all experiments were conducted at COD 150 mg/l, pH 7.5 and 30-35 oC (room temperature). For each carbon source, initial nitrate were controlled at 50, 100, 150, 200 and 300 mg/l. At 200 mg/l sodium acetate, 99.3% nitrate was reduced at 45.26 mg /g MLVSS-h. Using wastewater from canned fruit juice plant nitrate reduction was 91.8% and the reduction rate was 37. mg /g MLVSS-h. Nitrate reduction by using biodiesel waste was 81.76% nitrate reduction at 35.02 mg /g MLVSS-h. was obtained. Therefore wastewater can be use as an electron donor for denitrification efficiently though lower both rate and % removal than sodium acetate.