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Phuwadol Bangrak. Cloning of Bowman-Birk Inhibitor Isolated from Bambara Groundnut (Vigna subterranea). (). WALAILAK UNIVERSITY. CENTER FOR LIBRARY RESOURCES AND EDUCATIONAL MEDIA. : , 2011-10-12.
Cloning of Bowman-Birk Inhibitor Isolated from Bambara Groundnut (Vigna subterranea)
Name: Phuwadol Bangrak
Email :
bphuwado@wu.ac.th
keyword:
Bowman-Birk inhibitor
;
RT-PCR
;
RACE
Abstract:
Bowman-Birk inhibitors (BBIs) are a member of serine proteinase inhibitors which inhibit trypsin and chymotrypsin
or elastase. Legume BBIs played the important roles in plant defense mechanisms and also exhibited anticarcinogenic and
anti-inflammatory activity. Full-length cDNA of bambara groundnut (Vigna subterranea) BBI or BBI-Vs was isolated from
immature seed by reverse transcription PCR (RT-PCR) and rapid amplification of complementary DNA ends (RACE)
techniques. The open reading frame of BBI-Vs is 366 bp encoded 121 amino acids with predicted molecular mass of
approximately 13.2 kDa. Its amino acid sequences shows high homology with double-headed trypsin inhibitor of Phaseolus
microcarpus (88%) and consists of two reactive sites (CTKSMPPQC and CTFSIPAQC) which are responsible for trypsin
and chymotrypsin inhibition, respectively. The recombinant protein BBI-Vs will be expressed in Escherichia coli or Pichia
pastoris to investigate the biochemical properties.
WALAILAK UNIVERSITY. CENTER FOR LIBRARY RESOURCES AND EDUCATIONAL MEDIA
Address:
NAKON SI THAMMARAT
Email:
clm@wu.ac.th
Created:
2011-10-12
Modified:
2016-06-14
Issued:
2554-10-12
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Phuwadol Bangrak