Molecular cloning and characterization of Mulberry (Morus spp) stilbene synthase gene involved in resveratrol production

Name: Benyapa Manosan.

LCSH: Kasetsart University -- Theses. M.S. (Biochemistry) 2023

Classification :.LCCS: QK495.M73

LCSH: Kasetsart University. -- Department of Biochemistry -- Theses.

LCSH: Mulberry -- Genetics.

LCSH: Plant tissue culture.

LCSH: Gene expression.

LCSH: Plant metabolites.

Abstract: Resveratrol, a prominent secondary metabolite exclusive to phenolic plants, serves a pivotal role in responding to adverse conditions and finds extensive applications in medicinal contexts. This study focuses on the cloning and characterization of the stilbene synthase (STS) gene in mulberry (Morus spp.) and explores resveratrol quantity, along with the expression of resveratrol biosynthesis-related genes, under the influence of high temperature, UV-C, and their combination in mulberry callus. The 3 mulberry cultivars, namely Kamphaengsaen MB-42-1 (KPS), Buriram 60 (BR60), and Khunpai (KP), were used as plant models to identify the STS gene. As a result, the mulberry STS gene was successfully cloned, consisting of 1,176 bp, which encoded into 391 amino acid residues. Utilizing the KPS cultivar, callus induction was conducted under various stress conditions, including high temperature (40°C for 12 hours), UV-C exposure (15 minutes, followed by 6 hours recovery), and the combination of high temperature with UV-C. High-performance liquid chromatography (HPLC) analysis revealed a significant increase (2.17-fold) in resveratrol and a 1.66-fold increase in oxyresveratrol in response to high temperature. UV-C exposure (15 minutes, followed by 24 hours in darkness) exhibited the most significant accumulation of oxyresveratrol, showing a 2.33-fold increase. Furthermore, the expression of genes involved in resveratrol and oxyresveratrol biosynthesis (PAL, 4CL, STS, and CHS) was examined using qRT-PCR. UV-C prominently induced the expression of biosynthesis-related genes, while prolonged exposure to high temperature reduced their expression. Both high-temperature and UV-C were efficient in promoting resveratrol and oxyresveratrol accumulation in mulberry callus when incubated for extended periods, even if the contrast effect with gene expressions, suggesting the synergistic effect of high-temperature with UV-C. These findings provide insights for the development of transgenic plants with enhanced resveratrol and oxyresveratrol production, thereby improving the quality of mulberry

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Songwut Suramitr

Role: Thesis Advisor

Name: Nattanan T-Thienprasert

Role: Thesis Co-Advisor

Created: 2023

Modified: 2026-07-07

Issued: 2026-07-07

วิทยานิพนธ์/Thesis

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2566/benyapa-man-all.pdf

CallNumber: QK495.M73 .B46

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biochemistry

Grantor: Kasetsart University

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