The in vitro responses of tobacco and sugarcane tissue to various LED spectra

Name: Pattira Wiyasing

LCSH: Kasetsart University -- Theses. M.S. (Agricultural Biotechnology) 2022

Classification :.LCCS: SB273

LCSH: Kasetsart University. -- Interdisciplinary Graduate Program -- Theses

LCSH: Tobacco -- Propagation

LCSH: Sugarcane -- Propagation

LCSH: Plant tissue culture

LCSH: Tissue culture

Abstract: The objective of this research was to study the effect of the LED spectrum on tobacco and sugarcane tissue culture. Leaf blade and single node explants of tobacco was cultured on MS solid medium supplemented with 2 mg/L BA and incubated under various light conditions. The white (W-LG) and white with blue (WB) LEDs yielded the comparable results in shoots regeneration either from leaf blade and single node explants as compared with the one incubated under fluorescent light. The study of the LEDs spectra application for sugarcane tissue culture was performed in three sugarcane cultivars, KK3, LK92-11, and KPS01-12. Three steps of micropropagation were investigated including callus induction from young rolled-leaf, shoots regeneration from callus and tillering ability of a single plantlet. In general, the MS solid medium supplemented with 3 mg/L 2,4-D and 10% coconut water incubated in darkness for 8 weeks was good for callus induction. However, the W-LG, BR and RB LEDs could be used as their callus induction ability was not significant different from the control treatment that the callus was induced in darkness. When those calli were subjected to shoot regeneration, the white with red (WR) LED was the best light source for shoot induction. However, it was also revealed that, in KK3 and LK92-11 cultivars, calli induced under B or BR LEDs developed into shoots more efficient than the calli induced in darkness. In contrast, in KPS01-12 cultivar, the darkness induced calli regenerated well under WR LED. For the study of tillering ability, the WR LED was the suitable light condition to be used for tillers proliferation induction in all three cultivars tested.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Sermsiri Chanprame

Role: Thesis Advisor

Name: Sontichai Chanprame

Role: Thesis CO-Advisor

Created: 2022

Modified: 2025-07-27

Issued: 2025-07-27

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2565/pattira-wiy-all.pdf

CallNumber: SB273 .P38

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Agricultural Biotechnology

Grantor: Kasetsart University

©copyrights Kasetsart University

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