Identification of molecular mechanism controlling waterlogging tolerance and functional characterization of stress-regulated transcription factor genes in Vigna radiata

Name: Pimprapai Butsayawarapat

LCSH: Kasetsart University -- Dissertations. Ph.D. (Genetics) 2023

Classification :.LCCS: QK495.L52

LCSH: Kasetsart University. -- Department of Genetics -- Dissertations

LCSH: Mung bean -- Genetics

LCSH: Plants, Effect of ethylene on

LCSH: Transcription factors

LCSH: Plant hormones

LCSH: Waterlogging (Soils)

Abstract: Waterlogging (WS) is one of abiotic stresses that negatively impacts plants by limiting oxygen availability. This study delves into the molecular mechanisms that help plants survive WS, with a specific focus on ethyleneresponsive factor (ERF) transcription factors. ERFs play crucial roles in regulating plants adapt to flooding stress. Vigna, including zombi pea and mungbean, holds agricultural significance due to its versatility and valuable contributions. Zombi pea exhibits significant genetic diversity, which is valuable for breeding programs aimed at enhancing its resilience and nutritional properties. On the other hand, mungbean is recognized for its rapid growth and short cultivation cycle, making it a critical component of crop rotation systems that improve soil health. Their seeds are crucial for global food security due to their wide culinary versatility and widespread consumption. The research compared two zombi pea varieties, “A408” and “Bali”, highlighting the remarkable WS tolerance of “A408” through increased lateral root (LR) formation and sustained chlorophyll content. Transcriptomic analysis revealed various metabolic processes contributing to WS tolerance. Our exploration into the ERF subfamily X (ERFX), derived from the zombi pea transcriptome, revealed valuable insights. We applied this concept to improve WS tolerance of mungbean. By focusing on the ERF subfamily X-134 (ERFX-134) gene, selected from among the ERFX genes obtained from mungbean, we observed a significant increase in LR formation in transgenic mungbean hairy root lines overexpressing ERFX-134. Overexpression of the ERFX-134 gene in Arabidopsis enhances WS tolerance by promoting root and plant growth. These findings suggest that the ERFX-134 gene plays a pivotal role in regulating root growth and facilitating the adaptation to WS. Future research is necessary to gain a better understanding of how the ERFX-134 gene functions in response to WS in mungbean. Exploring the consequences of upregulating the ERFX-134 gene within mungbean during WS presents an exciting avenue for research. This investigation could shed light on the effects it may have on the complex molecular pathways governing the WS response, employing advanced techniques like RNA-seq and ChIP-seq for an in-depth exploration. The knowledge from this study has the potential to enhance mungbean's WS tolerance, leading to increased crop productivity.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Piyada Juntawong

Role: Thesis Advisor

Name: Supachai Vuttipongchaikij

Role: Thesis CO-Advisor

Created: 2023

Modified: 2025-07-27

Issued: 2025-07-27

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2566/pimprapai-but-all.pdf

CallNumber: QK495.L52 .P56

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Genetics

Grantor: Kasetsart University

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