Dissecting metabolic behavior of limosilactobacillus reuteri KUB-AC5 through genome and transcriptome data integration

Name: Theeraphol Jatuponwiphat

LCSH: Kasetsart University -- Dissertations. Ph.D. (Bioscience) 2021

Classification :.LCCS: QR82.L3

LCSH: Kasetsart University. -- Faculty of Science -- Dissertations

LCSH: Lactic acid bacteria

LCSH: Probiotics

LCSH: Bacteriocins

LCSH: Bioinformatics

LCSH: Gastrointestinal system -- Microbiology

Abstract: Limosilactobacillus reuteri KUB-AC5 is a heterofermentative lactic acid bacteria isolated from the chicken intestine and widely used as a chicken probiotic. Its genes and functions in terms of probiotic properties are still largely unknown. However, the underlying metabolic responses to growth and inhibition effects of L. reuteri KUB-AC5 do not adequately characterize a preferred carbon source and growth phase. This study aimed to perform genome sequencing of L. reuteri KUB-AC5 and further accomplished comparative analysis with overall identifying its unique/shared metabolic genes and functions across L. reuteri genomes, as well as investigate transcriptome profiling of L. reuteri KUB-AC5 revealing global metabolic responses when alteration of carbon sources and growth phases. As a result, a genome sequence of L. reuteri KUB-AC5 has a total length of 2,187,995 base pairs and a GC content of 39.29%. Based on metabolic annotation, 477 genes out of 2,196 protein-encoding genes were discovered to be involved in metabolism, particularly carbohydrates, cofactors, and vitamins. Interestingly, cobalamin (vitamin B12) and folate (vitamin B9) were proposed to be related to the probiotic properties of L. reuteri KUB-AC5. In terms of gene cluster identification, a putative unique exopolysaccharide (EPS) biosynthetic gene cluster was also discovered in this KUB-AC5 strain. In the context of a bacteriocin-producing strain, the L. reuteri KUB-AC5 genome reveals that its genes are involved in immune, regulatory, and transporter functions. Interestingly, L. reuteri KUB-AC5 grown in sucrose culture performed the best in terms of fast growth and inhibition of Salmonella Enteritidis S003 growth. The results of the transcriptome profiling and reporter proteins/metabolites analysis revealed that amino acid transport via ABC systems, as well as sucrose metabolism and transport, are key metabolic responses during the Logarithmic (L)-phase of L. reuteri KUB-AC5 growth. Considering the Stationary (S)-phase discovered potential reporter proteins/metabolites involved in carbohydrate metabolism e.g., levansucrase and levan. Levansucrase and levan were found to be promising candidates for inhibiting the effects of L. reuteri KUBAC5. This study serves to guide on how L. reuteri KUB-AC5 is used as probiotics for impact on host health. This study has a broader impact on designing efficient probiotics for industrial foods and feeds.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Wanwipa Vongsangnak

Role: Thesis Advisor

Name: Massalin Nakphaichit

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-26

Issued: 2025-07-26

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/theeraphol-jat-all.pdf

CallNumber: QR82.L3 .T44

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Bioscience

Grantor: Kasetsart University

©copyrights Kasetsart University

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