Functional properties and structure characterization of sulfated galactan from Gracilaria spp. (G. lemaneiformis and G. fisheri)

Name: Khosook Chumsook

LCSH: Kasetsart University -- Theses. M.S. (Fishery Science and Technology) 2023

Classification :.LCCS: QK569.G8

LCSH: Kasetsart University. -- Faculty of Fisheries -- Theses

LCSH: Gracilaria

LCSH: Red algae

LCSH: Galactans

LCSH: Agar

LCSH: Antioxidants

Abstract: The high potential for agar production as genus Gracilaria of red seaweed, likes G. lemaneiformis from China and G. fisheri from Thailand also has several beneficial biological activities from sulfated galactans (SGs) in their cell wall. Aims of this study are extracted sulfated galactans and determine their chemical composition, structure, and determine the functional properties. Polysaccharides of seaweed samples (T for G. fisheri from Songkhla, Thailand, and N, C, S for G. lemaneiformis from different locations of Rongcheng, Rizhao, and Putian) were extracted by hot water, and dialyzed to obtain SGs, then compared their chemical and physical characteristics. For FTIR results, all samples showed similar infrared spectra with characteristic signals of carbohydrate regions, and the position of the sulfate group for sulfated polysaccharides. For antioxidant activity, C sample exhibited the significantly highest dose-dependent DPPH radical scavenging capacity, while the highest ferrous chelating ability was found in N sample. Besides, S sample had the highest reducing power ability. Moreover, SGs from T and C samples decreased PPAR-γ and C/EBP-α gene expression rather with increasing intensity but lower than Quercetin, which was a flavonoid as a positive control. C, S and T samples can significantly reduce the accumulation of triglycerides compared to control. SGs from T and S samples showed the highest percentage of ACE inhibition among the others. Likewise, T sample also exhibited the highest both α-Amylase inhibitory and lipase inhibitory activity. SGs from T, N, and C samples showed greater than 75% cell viability at all concentration and S sample had increased cell viability compared to the control sample. For a study of NO production on LPS-induced RAW 264.7 cell, S sample decreased NO production with dose-dependent manner as anti-inflammatory properties. However, the samples that had no anti-inflammatory effects were further studied by treated with RAW 264.7 cells without LPS to screen the immune-stimulation activity. Both T and C samples increased NO production with dose-dependent manner, and T had greater than C sample at all concentration. For N sample had no activities on antiinflammatory and immune-stimulation assays. These studies provide the potential application of sulfated galactans from G. fisheri and G. lemaneiformis as natural agents in functional food and pharmaceutical industries. These seaweed samples should be purified to obtain more effective substance and examined in other in vitro and in vivo methods to ensure all results.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Jantana Praiboon

Role: Thesis Advisor

Name: Xiaoting Fu

Role: Thesis CO-Advisor

Created: 2023

Modified: 2025-07-24

Issued: 2025-07-24

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2566/khosook-chu-all.pdf

CallNumber: QK569.G8 .K58

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Fishery Science and Technology

Grantor: Kasetsart University

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