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Kantharakorn Macharoen. Effects of cultivation temperature on alpha-amylase production from wild-type rice callus suspensions. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2025.
Effects of cultivation temperature on alpha-amylase production from wild-type rice callus suspensions
Name: Worapicha Daungtongcome
Organization :
King Mongkut's University of Technology Thonburi. Faculty of Engineering
Name: Kantharakorn Macharoen
Organization :
King Mongkut's University of Technology Thonburi. Faculty of Engineering
Email :
kantharakorn.mac@kmutt.ac.th
keyword:
Rice cell cultures
Abstract:
α-Amylase is a key enzyme that hydrolyzes starch into smaller sugars, with applications
in food, pharmaceuticals, and biofuels. The objective of this research is to study the
effects of cultivation temperature on rice cell growth and the production of α-amylase.
Rice callus (Oryza sativa subsp. japonica cv. Nipponbare) suspensions were cultured for
14 days at 140 rpm in 1-L shake flasks under three different temperatures: 27°C, 32°C,
and 37°C. Samples were taken and centrifuged to separate spent media (supernatant) and
rice callus pellet on days 0, 2, 4, 6, 8, 10, 12, and 14. The α- amylase activity was
measured using the DNS (3,5-dinitrosalicylic acid) method. The results indicated that
the amount of α-amylase in the spent media was approximately 45 times higher than in
the cell extract at all three tested temperatures. The optimal conditions for α-amylase
activity were found to be at 37°C and pH 7.0.The highest α-amylase activity in the spent
media was found at 37°C (1.02 U/ml), significantly higher than those of 32°C (0.90
U/ml) and 27°C (0.86 U/ml), when one unit of enzyme (U) liberates 1.0 mg of maltose
from starch in 3 minutes at pH 7.0 and 37°C. During the final days of cultivation, α-
amylase is produced to break down stored starch into sugars, compensating for the
decreased sugar levels and maintaining energy balance. This approach has the potential
to be applied in industry as an alternative for future production processes
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2025
Modified:
2025-07-22
Issued:
2025-07-22
บทความ/Article
application/pdf
BibliograpyCitation :
In Thai Institute of Chemical Engineering and Applied Chemistry and Khon Kaen University. Faculty of Engineering. The 34th Thai Institute of Chemical Engineering and Applied Chemistry International Conference (TIChE 2025) (TIChE 2025 TIChE-BE-15). Bangkok : Thai Institute of Chemical Engineering and Applied Chemistry, 2025
eng
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0.030549 วินาที
Worapicha Daungtongcome
| Title | Contributor | Type |
|---|---|---|
| Effects of cultivation temperature on alpha-amylase production from wild-type rice callus suspensions
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Worapicha Daungtongcome;Kantharakorn Macharoen | บทความ/Article |
Kantharakorn Macharoen
| Title | Contributor | Type |
|---|---|---|
| Mathematical modeling of growth kinetics and recombinant butyrylcholinesterase production of transgenic rice cell suspension cultures
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Paradon Sakornpanich;Kantharakorn Macharoen | บทความ/Article | |
| Rice Bran Wax as a renewable resource for biolubricant formulation
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Phee Petchruarn;Kantharakorn Macharoen;Jindarat Pimsamarn | บทความ/Article | |
| Effects of cultivation temperature on alpha-amylase production from wild-type rice callus suspensions
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Worapicha Daungtongcome;Kantharakorn Macharoen | บทความ/Article |