Effects of SoMIPS and SoDREB gene from Sugarcane and their conditions on salt and water deficit tolerance of transgenic Tobacco

Name: Suwannarat Savaengrujitham

LCSH: Kasetsart University -- Theses. M.S. (Agricultural Biotechnology) 2022

Classification :.LCCS: SB231

LCSH: Kasetsart University. -- Interdisciplinary Graduate Program -- Theses

LCSH: Sugarcane -- Effect of salts on

LCSH: Sugarcane -- Effect of drought on

LCSH: Tobacco -- Genetics

LCSH: Soil salinization

LCSH: Polymerase chain reaction

Abstract: Sugarcane planting areas in Thailand, especially in the central and northeastern regions are experiencing saline soil problems and causing the sugarcane growth and yield to decrease. It is likely that the saline soil areas are expanding widely. This research aimed to study the effects of gene transfusion and investigation the expression of the transgenes upon salt and water deficit stress by transferring and expressing the transgenes, SoMIPS and SoDREB gene into tobacco plants. In addition, the condition of the transgenes in transgenic tobacco that may affect the levels of salt and water deficit tolerant was also studied. SoMIPS gene from wild sugarcane and SoDREB from interspecific hybrid sugarcane were transferred to different tobacco plants. The progenies that contained homozygous or heterozygous genes were detected and used for gene expression and salt and water deficit tolerant experiments. The relationship between the degree of tolerance and the status of the transgenes in the plant were also investigated. The expression levels of SoMIPS and SoDREB in the transgenic tobacco subjected 150 mM NaCl or 2.5% PEG 6000 and 150 mM NaCl with 2.5% PEG 6000 for 7 days in vitro were also compared. It was indicated that the expression of the SoMIPS gene alone and the combination of both genes in transgenic tobacco received 150 mM NaCl with 2.5% PEG 6000 as well as the transgenic tobacco containing SoDREB gene received 2.5% PEG 6000 increased steadily from day 0 to day 7. However, at the 150 mM NaCl condition, the expression of both genes had increased until day 5 and decreased at day 7. When salt stress and water deficits were more severe and last for a longer period of time, the expression of SoMIPS, SoDREB, or SoMIPS + SoDREB increased in the roots and leaves of transgenic tobacco plants. These transgenic tobacco were more resistant to salt stress and dehydration than wild type tobacco (control). In greenhouse condition, the transgenic tobacco plants had larger and thicker leaves than the wild type plant. Among other things, they grew more quickly than the wild type tobacco at the same age. The four tobacco lines were cultivated in different settings, and it was shown that those with SoMIPS or SoDREB as their sole transgene were more tolerate to salt stress and water deficit stress than those with both genes. Measuring the SPAD units, transgenic tobacco plants which contained SoMIPS, SoDREB and SoMIPS+SoDREB had similar amounts of SPAD units under stress conditions while the wild type tobacco plants whose SPAD units continued to decline under more stressful conditions and over longer periods of time. The leaf total water potential of wild type tobacco was lower than that of SoMIPS, SoDREB and SoMIPS+SoDREB transgenic plants. In the salt stress experiment, The leaf total water potential of the SoMIPS+SoDREB containing plant was higher than that of other lines as in dehydration stress as well as salt stress plus dehydration. Overall, the experiment showed that the transgenic tobacco with the the two genes, SoMIPS+SoDREB, expressed their genes more than the tobacco with a single gene, and showed a higher degree of salt and/or water deficit tolerance.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Sontichai Chanprame

Role: Thesis Advisor

Name: Sermsiri Chanprame

Role: Thesis CO-Advisor

Created: 2022

Modified: 2025-07-22

Issued: 2025-07-22

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2565/suwannarat-sav-all.pdf

CallNumber: SB231 .S89

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Agricultural Biotechnology

Grantor: Kasetsart University

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