Identification and inhibition bacteria causing an odor of Rubber cup

Name: Saifa Pumloifa

LCSH: Kasetsart University -- Theses. M.S. (Genetic Engineering and Bioinformatics) 2021

Classification :.LCCS: TS1892

LCSH: Kasetsart University. -- Interdisciplinary Graduate Program -- Theses

LCSH: Rubber

LCSH: Bacteria

LCSH: Polymerase chain reaction

LCSH: Anti-infective agents

LCSH: Gas chromatography

The rubber industry is one of the major industries in Thailand. However, many volatile fatty acids (VFA) are the cause of undesirable odors which come from the digestion of organic substances contained in natural rubber latex by microbes. In this study, we aimed to identify the microbes which cause the undesirable odors in natural rubber. Bacterial identification was performed by 16S rRNA amplification. All 16S rRNA sequences was generated a phylogenetic tree by using the Maximum Likelihood (ML) and Neighbor joining (NJ) method. Interestingly, Enterobacter cloacae and Klebsiella pneumoniae were predominantly identified that are evolutionarily related to odor-producing strains. Acetic acid, citric acid, and sodium benzoate have been reported for the odor-producing strains inhibition. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were performed to determine their effect. Acetic acid, citric acid, and sodium benzoate demonstrated MIC at 3.13-25 mM, 3.13-50 mM, and 12.5-200 mM, respectively and MBC at 6.25-100 mM, 3.13-100 mM, and 12.5-200 mM, respectively. After that natural rubber cup prepared from 5% formic acid and 5% sulfuric acid with 30 mM acetic acid and 200 mM sodium benzoate and then the rubber were evaluated for odor sensory test, GC-MS and quantity of bacteria. The results shown slightly odor and less gas profile and copy number of bacteria at 3,365,507 copy/g for quantitative PCR technique of rubber coagulated with 5% formic acid and 200 mM sodium benzoate. Therefor sodium benzoate has potential for natural rubber coagulant.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: https://www.lib.ku.ac.th/KUthesis/2564/saifa-pum-all.pdf

Role: Thesis Advisor

Name: Sasimanas Unajak

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-21

Issued: 2025-07-21

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/saifa-pum-all.pdf

CallNumber: TS1892 .S25

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Genetic Engineering and Bioinformatics

Grantor: Kasetsart University

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