Anti-bacterial and anti-cancer activities of green-synthesized Zinc Oxide nanoparticles from crude extract of Banana peels

Name: Jittiporn Ruangtong

LCSH: Kasetsart University -- Theses. M.S. (Biochemistry) 2018

Classification :.LCCS: RS431.A64

LCSH: Kasetsart University. -- Department of Biochemistry -- Theses

LCSH: Antibacterial agents

LCSH: Antineoplastic agents

LCSH: Zinc oxide

LCSH: Bananas -- Utilization

LCSH: Plant extracts

LCSH: Nanoparticles

Abstract: Presently, zinc oxide nanoparticle (ZnO NP) is an essential complement in several food industries and agriculture. Moreover, they are reported to contain board antibacterial activities and anticancer activity. However, the chemical synthesis of ZnO NPs is expensive and requires high pressure. Moreover, the chemical synthesis generates toxic chemicals to environments such as H2S, metallic precursor and toxic template. Therefore, this study aimed to synthesize ZnO NPs from a reaction of zinc acetate with different concentrations of banana peeled crude extract and examine the effects of ZnO NPs on growth of bacteria and cell viability of cancer cell lines. The results indicated that ZnO NPs could be synthesized from a mixture between zinc acetate and banana peeled crude extract in the appropriate condition. The higher concentration of zinc acetate resulted in higher yield of the obtained powder and larger sizes of ZnO NPs. XRD resulted also suggested that a higher concentration of banana peeled crude extract generated ZnO NPs with higher purity. Results from agar disc diffusion and bacterial growth assay revealed that synthesized ZnO NPs exhibited antibacterial activity against B.subtilis, S.epidermis and especially in E.coli. The best antibacterial activity was observed in ZnO NP 0.2 M2000 with IC50 against B.subtilis that equals to 0.6601 mg/mL. From MTT assay, the results confirmed that the newly synthesized ZnO NPs drastically inhibited cell viability of liver cancer cells (HepG2), skin cancer cells (A431) and colon cancer cells (SW620) without affecting normal cells (Vero). The best anti-cancer activity was observed in ZnO NPs 0.2 M-2000 with IC50 equals to 31.92 µg/mL on SW620 cell. Therefore, these studies firstly reported potential usage of the green-synthesized ZnO NPs for development of anti-bacterial substances or anticancer drugs. Moreover, this study demonstrated the opportunity to increase values of natural products.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Nattanan T-Thienprasert

Role: Thesis Advisor

Name: Pichamon Kiatwuthinon

Role: Thesis CO-Advisor

Created: 2018

Modified: 2025-07-15

Issued: 2025-07-15

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2561/jittiporn-rua-all.pdf

CallNumber: RS431.A64 .J58

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biochemistry

Grantor: Kasetsart University

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