Location of lipid components among centrifugation fractions of hevea brasiliensis latex

Name: Saowalak Jantarasunthorn

LCSH: Kasetsart University -- Theses. M.S. (Biochemistry) 2021

Classification :.LCCS: QK495.E9

LCSH: Kasetsart University. -- Department of Biochemistry -- Theses

LCSH: Hevea brasiliensis

LCSH: Rubber

LCSH: Latex

LCSH: Lipids

LCSH: Coagulation

Abstract: Lipid components of both whole latex and dry rubber have been previously characterized in order to relate their presence and the unique property of natural rubber. Nevertheless, the exact location of those studied compounds in latex and how they associated with NR was not well elucidated. Therefore, the aim of this work is to study lipid location, lipid content and classify lipids of the four Hevea latex fractions (cream, skim, serum and lutoid) in order to understand better their roles on NR quality. Fresh latex from RRIM600 and PB235 clones which are known for their different lipid composition and physical properties were used. Four fractions were obtained through four steps centrifugation condition. The fresh latex fraction was subjected to freeze dried prior lipid extraction. The method used for fresh latex lipid extraction was not suitable for cream and skim fractions thus the method for dry rubber extraction was applied. The lipid content of each fraction showed that lutoid contained the highest amount of lipids in both clones (10.17 – 13.91% w/w dry matter). The lesser amount of lipids was detected in skim (RRIM600; 4.15% and PB235; 6.22%) and cream (RRIM600; 1.75% and PB235; 2.90%). Taking into account the relative proportion of each fractions in fresh latex, though lipid content of cream was the lowest on a dry weight basis compared to other fractions its largest proportion in latex compensated the total lipid quantity. Therefore, the main location of lipids in latex from both clones was cream fraction. In terms of qualitative analysis, the main difference was often observed between which is a vacuole like structure and the rubber particle fractions (cream and skim). This fully characterized lipid composition by different chromatography techniques (TLC, SPE, GC-FID/MS and HPLC-MS) permitted to use as the database to further study the relationship between lipids and natural rubber properties.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Sumallika Morakul

Role: Thesis Advisor

Name: Laurent Vaysse

Role: Thesis CO-Advisor

Name: Siriluck Liengprayoon

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-10

Issued: 2025-07-10

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/saowalak-jan-all.pdf

CallNumber: QK495.E9 .S28

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biochemistry

Grantor: Kasetsart University

©copyrights Kasetsart University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	saowalak-jan-all.pdf	3.97 MB

ใช้เวลา

0.036054 วินาที