Identification of Trehalose-6-phosphate synthase in sugarcane

Name: Saruta Manitkul

LCSH: Kasetsart University -- Theses. M.S. (Agricultural Biotechnology) 2019

Classification :.LCCS: TP414

LCSH: Kasetsart University. -- Interdisciplinary Graduate Program -- Theses

LCSH: Trehalose

LCSH: Sugarcane -- Breeding

LCSH: Sugarcane -- Drought tolerance

LCSH: Gene expression

Abstract: Sugarcane (Saccharum officinarum L.) is a major crop for sugar and fuel production. Drought stress directly affects to yield, generally resulting in low productivity. Trehalose, a non-reducing sugar, was found in many plants as an osmoticum solution to control osmotic potential in cells and to maintain cell water balance. Trehalose-6-phosphate synthase (TPS) genes play a role in the process of trehalose biosynthesis and involved in abiotic stress tolerance mechanism in plants. This study aimed to identify the expression of Trehalose-6- phosphate synthase genes, STPS1, and STPS2, in sugarcane. Two sugarcane cultivars, KPS 94-13 and KPS 01-11-6, were multiplied and rooted by in vitro culture technique. Water deficit stress was induced by adding polyethylene glycol (PEG) 6000 in the MS medium containing NAA 5 mg/L at 16% and 28% (v/w) for 24, 48, 72, 96 and 120 hours. Water stress caused the morphological change and reduction in the total leaf water potential and osmotic potential in both sugarcane cultivars. KPS 94-13 showed less yellowing and significantly less declines in the total leaf water potential and the osmotic potential than KPS 01-11-6. Leaves and roots were collected from each stress condition for total RNA extraction and cDNA synthesis. The STPS1 and STPS2 genes were expressed in leaf and root tissues in both cultivars. The level of expression of STPS1 and STPS2 genes in KPS 94-13 were observed initially and gradually increased under long-term exposure to stress. In KPS 94-13, the STPS1 and STPS2 expression were up-regulated and showed higher levels than control and KPS 01- 11-6 in both leaf and root tissues. The full-length cDNA of STPS1 and STPS2 genes were cloned and identified, the full-length cDNA of STPS1 gene consisted of 2,553 bp and STPS2 gene was 2,731 bp. The phylogenetic analysis and protein structure analysis showed that the STPS1protein is in Class I TPS. STPS1 protein contains 15 motifs and 2 domains. The STPS2 protein contains 15 motifs and 3 domains and belongs in Class II TPS.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Nongluk Teinseree

Role: Thesis Advisor

Created: 2019

Modified: 2025-07-07

Issued: 2025-07-07

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2562/saruta-man-all.pdf

CallNumber: TP414 .S27

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Agricultural Biotechnology

Grantor: Kasetsart University

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