Analysis evidence of dramatic sterility in F1 male hybrid catfish [male clarias gariepinus (burchell, 1822)x female C macrocephalus (Gunther, 1864)] and complete mitochondrial genome characterization of mouthbrooding fighting fishe, betta apollon and Bsimplex (Teleostei Osphronemidae)

Name: Jatupong Ponjarat

LCSH: Kasetsart University -- Theses. M.S. (Genetics) 2019

Classification :.LCCS: QL637.9.S5

LCSH: Kasetsart University. -- Department of Genetics -- Theses

LCSH: Catfishes -- Genetics

LCSH: Betta

LCSH: Mitochondrial DNA

Abstract: Sterile hybrid animals exhibit spermatogenic disruptions with a decreased number and/or malformation of mature sperms. F1 hybrid catfish (2n = 55) is an important cultured fish derived from male North African catfish [Clarias gariepinus (Burchell, 1822); 2n = 56] and female bighead catfish [C. macrocephalus (Günther, 1864); 2n = 54]. Our observations in the F1 male hybrid showed abnormal morphology of testes with small size, while meiotic configuration indicated that meiosis succeeded in the early pachytene stage but failed to progress beyond the diplotene-diakinesis stage in primary spermatocytes. This suggests the presence of a number of degenerated spermatocytes. Histological examination recorded no postmeiotic cells and spermatozoa in F1 male hybrid testes. A high frequency of apoptotic testicular cells was also present in the F1 male hybrid, as shown by histochemical results for activated Caspase-3 and TUNEL assays. Low expression levels of Caspase-3, p53 and MCL1 (isoform 2), which activate pro-apoptotic signals in the development process, were also observed in both parental species, but significantly high expression levels were observed in the F1 male hybrid. By contrast, the state of apoptosis as BCL2 inhibits the actions of pro-apoptotic proteins. These results collectively suggest that sterility in male hybrids is caused by spermatogenic disruptions in the pachytene stage, resulting from the failure of homologous chromosome pairing due to chromosomal incompatibility between parental genomes, and subsequent elimination by apoptosis leading to spermatogenic breakdown in the F1 male hybrid. And for the other study complete mitochondrial genomes (mitogenomes or mtDNA) of Betta apollon and B. simplex were determined to support systematic and conservation programs. Mitochodrial genome sequences were 16,536 and 16,549 bp in length with slight AT bias (56.68% and 56.60% respectively), containing 37 genes with identical order to most teleost mitogenomes. Phylogenetic analysis of B. apollon showed a closer relationship with B. simplex, and grouped with B. pi as a monophyletic clade of mouthbrooders. Results will facilitate evolutionary studies, species diversity and conservation management in fighting fishes.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Kornsorn Srikulnath

Role: Thesis Advisor

Name: Surin Peyachoknagul

Role: Thesis CO-Advisor

Name: Narongrit Muangmai

Role: Thesis CO-Advisor

Created: 2019

Modified: 2025-07-02

Issued: 2025-07-02

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2562/jatupong-pon-all.pdf

CallNumber: QL637.9.S5 .J38

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Genetics

Grantor: Kasetsart University

©copyrights Kasetsart University

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