Identification of EgBADH and EgMIPS of oil palm (Elaeis guineensis) under water deficit stress

Name: Jirasak Wichasawasdi

LCSH: Kasetsart University -- Dissertations. Ph.D. (Agricultural Biotechnology) 2019

Classification :.LCCS: SB299.P3

LCSH: Kasetsart University. -- Interdisciplinary Graduate Program -- Dissertations

LCSH: Oil Palm -- Effect of stress on

LCSH: Oil Palm -- Water deficit stress

LCSH: Oil Palm -- Genetics

LCSH: Oil Palm -- Physiology

LCSH: Oil Palm -- Growth

Abstract: Water deficit stress is an important problem in oil palm (Elaeis guineensis Jacq.) production. Betaine aldehyde dehydrogenase (BADH) and Myo-inositol phosphate synthase (MIPS) gene have important roles in water deficit stress response in many plants. The objectives of this study were to clone the full-length of EgBADH2 and EgMIPS gene from Tenera oil palm and to study the expression pattern of both genes and physiological data during water deficit stress. For oil palm seedlings, the water deficit stress was induced by 30% PEG 6000. Physiological data were collected from water deficit stressed oil palm seedlings for 28 days. The results showed the lowest of ψt was about -1,400 kPa in the stressed plant while the control plant was about -500 kPa at 28th day. The ψπ in leave tissue of the control was lower than that of the under water deficit. The Ψp of the control seedling was higher than that of water deficit stressed oil palm seedlings. The Cs of oil palm seedlings that received water deficit stress was higher than that of the control treatment. The stress group had Ødark lower than 0.8 which was lower than the control group. The ETR was reduced in stressed plants and it was lower than the control. For cloning and sequencing analysis of MIPS and BADH gene in oil palm, the results showed that the full length of EgBADH2 and EgMIPS were 1,512 and 1,533 bp and encoded a protein of 503 and 510 amino acids, respectively. The expression patterns of both genes were down-regulated pattern when oil palm received water deficit stress. The cloned genes were transformed into a tobacco plant via Agrobacterium-mediated transformation for complementary study. The transgenic tobacco line G, H, M were transformed with EgBADH gene and were subjected to water deficit stress at 0%, 5%, 7.5 %, and 10% PEG6000 for 5 days. All transgenic tobacco lines had higher ψp values than the wild type at 10% PEG 6000. The transgenic tobacco line A, B, K were transformed with EgMIPS gene and were subjected to water deficit stress as same as previous stress condition. The result showed that „line K‟ had the highest of ψt and was statistically significant difference with wild type (P<0.05) at 5 days after receiving 10% PEG 6000. At 5 days of 7.5% PEG 6000 induced stress, all transgenic lines had higher ψp than that of wild type. The relative expression of EgMIPS gene in leaves of the transgenic tobacco „line B‟ received 5% PEG6000 for 5 days was the highest expression of 1,365X with significant difference from wild type. The expression of this gene in leaves was an up-regulation type. In roots, the expression level of the gene under non-stress condition was the highest expression of 2,645X. The relative expression of the EgMIPS was down regulation in roots under water deficit stress.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Sontichai Chanprame

Role: Thesis Advisor

Name: Anuruck Arunyanark

Role: Thesis CO-Advisor

Created: 2019

Modified: 2025-07-01

Issued: 2025-07-01

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2562/jirasak-wic-all.pdf

CallNumber: SB299.P3 .J57

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Agricultural Biotechnology

Grantor: Kasetsart University

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