Differential expression of ethylene signal transduction and receptor genes during ripening and dehiscence of durian (Durio zibethinus Murr) fruit

Name: Monthathip Thongkum

LCSH: Kasetsart University -- Dissertations. Ph.D. (Horticulture) 2018

Classification :.LCCS: SB379.D8

LCSH: Kasetsart University. -- Department of Horticulture -- Dissertations

LCSH: Durian -- Ripening

LCSH: Durian -- Genetic aspects

LCSH: Gene expression

LCSH: Ethylene

Abstract: Durian (Durio zibethinus Murr) cv. Monthong is a climacteric fruit and the rise of respiration and ethylene production coincides with ripening. Biochemical changes are taken place in the durian pulp, while the husk is dehiscent during ripening. Rapid ripening is a significant problem that limits the shelf life of durian, with ethylene having a major impact on the regulation of this event. Durian treated with ethephon ripened 3 days after treatment with increased total soluble solids, ethylene production and decreased pulp firmness compared to the control fruit. 1-methylcyclopropene (1-MCP) treatment delayed ripening by up to 9 days with inhibited accumulation of total soluble solids, pulp color change, ethylene production and softening. While fruit dehiscence started a week after harvest, was hastened by ethephon treatment, and delayed by 1-MCP. 1-MCP treatment delayed dehiscence for 5 days from day 7 to day 12 compared with untreated fruit held at 25°C. Two ethylene receptors (DzETR1 and DzETR2), a CTR1-like protein (DzCTR1) and two ethylene insensitive-like proteins (DzEIL1 and DzEIL2) from durian fruit were isolated. The transcript levels of these genes were investigated during this process using qPCR. All candidate genes slightly down-regulated during fruit development, showed a negative correlation with fruit expansion. During fruit ripening, the transcript abundances of signal transduction genes increased the transcripts of all genes were activated by ethephon treatment and inhibited by 1-MCP treatment. The transcription of genes expression showed positively related with increase of ethylene production, TSS and pulp color. In dehiscence zone of the husk, ethylene signal transduction genes were constitutively expressed. All genes had slightly higher transcript abundances. In early stage of dehiscence, all genes were inhibited by 1-MCP treatment and then increased rapidly in expression found on day 9 concomitant with husk dehiscence. The promoters of the DzACS and DzETR2 genes were also isolated and analyzed their activation by using transient expression in tobacco leaves. It was found that members of the MADS box transcription factor families activated the DzACS promoter, while EIL1, EIL2 and EIL3 transcripton factors strongly activated the DzETR2 promoter. The results suggested that ethylene biosynthesis and ripening of durian pulp is induced by endogenous ethylene, via the regulation of DzACS and DzETR2 by MADS and EIL transcription factors.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Emeritus Saichol

Role: Thesis Advisor

Name: Wachiraya Imsabai

Role: Thesis CO-Advisor

Name: Parichart Burns

Role: Thesis CO-Advisor

Created: 2018

Modified: 2025-07-01

Issued: 2025-07-01

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2561/monthathip-tho-all.pdf

CallNumber: SB379.D8 .M66

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Horticulture

Grantor: Kasetsart University

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