Cloning and characterization of NHXI gene of sugarcane

Name: Tanawan Promkhlibnil

LCSH: Kasetsart University -- Dissertations. Ph.D. (Agricultural Biotechnology) 2019

Classification :.LCCS: SB231

LCSH: Kasetsart University. -- Interdisciplinary Graduate Program -- Dissertations

LCSH: Sugarcane -- Breeding

LCSH: Gene expression

LCSH: Cloning

Abstract: Sugarcane is an economic crop for producing sugar but the plant is sensitive to saline soil. To solve this problem, salt tolerant sugarcane cultivar must be improved through breeding program. The objectives of this research were to clone and identify the NHX1 gene from three difference genotypes of sugarcane KPS94-13 (SoNHX1KPS), Biotech 2 (SoNHX1BIO) and wild sugarcane (SsNHX1WT) and to study the expression pattern of the gene in salt stress condition. It was found that the full length of the gene was 1,623 bp and encoded a polypeptide of 540 amino acid residues. There were 9 amino acids difference amoung the amino acid sequences deduced from the genes of the three sugarcane species. The phylogenetic tree analysis indicated that the three genes were genetic closely to AtNHX1. SoNHX1KPS and SoNHX1BIO were closely related to each other more than the SsNHX1WT. The analysis of hydrophobicity found 11 transmembrane domains in the polypeptide sequences deduced from the nucleotide sequences of the gene. Gene expression study of NHX1 in the three sugarcane subjected to 100 mM and 200 mM of NaCl for 5 days revealed that the expression patterns of the NHX1 gene were depended on the concentrations of NaCl. The expression levels of the gene in leaves and roots of wild sugarcane were higher than those of Biotech 2 and KPS94-13. For characterization of those genes by using transgenic tobacco and investigation of the gene expression in roots and leaves of the transgenic lines compared to non-transgenic line indicated that the genes were up-regurated by salt stress.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Sontichai Chanprame

Role: Thesis Advisor

Created: 2019

Modified: 2025-06-30

Issued: 2025-06-30

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2562/tanawan-pro-all.pdf

CallNumber: SB231 .T36

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Agricultural Biotechnology

Grantor: Kasetsart University

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