Developing database for perdiction of bioactive peptides derived from food product

Name: Thitima Panyayai

LCSH: Kasetsart University -- Dissertations. Ph.D. (Genetic Engineering) 2019

Classification :.LCCS: TX553.P7

LCSH: Kasetsart University. -- Interdisciplinary Graduate Program -- Dissertations

LCSH: Peptides

LCSH: Protein

LCSH: Peptide amphiphiles -- Formation

LCSH: Bioactive compounds

Abstract: Bioactive peptides derived from food are important sources for alternative medicine and possess therapeutic activity. Several biochemical methods have been achieved to isolate bioactive peptides from food, which are tedious works and labors load as well as time consuming. In silico approaches are the alternative methodologies applying in bioactive peptide production resulting in cost and time efficient. In this research, the platform and database have been developed assisting in bioactive peptide (BPs) discovery from food. In order to understand whole platform, the angiotensin Iconverting enzyme (ACE) was selected as the protein prototype to construct platform from 8000 tripeptides. This platform was successfully discover new tripeptides inhibitors including WCW, IWW, WWW, WWI and WLW. The inhibitory profiles of these 5 peptides against ACE activity were confirmed with experimental lab. Their IC50 are 49.50 ± 3.88 μM, 489.14 ± 8.84 μM, 536.02 ± 38.57 μM, 752.91 ± 41.89 μM and 1783 ± 0.113 μM, respectively. Therefore, our computational protocol could be considered as a new tool for identifying active peptide against ACE. Moreover, FeptideDB database was developed to assist in forecasting bioactive peptides from food by combining peptide cleavage tools and database matching. Furthermore, this application was able to predict the potential of cleaved peptides from ‘enzyme digestion module’ to identify new ACE (angiotensin converting enzyme) inhibitors using an automatic molecular docking approach. FeptideDB is freely available to researchers who are interested in exploring bioactive peptides. FeptideDB is accessed via http://www4g.biotec.or.th/FeptideDB/. Ultimately, FeptideDB is a computational aid for assessing peptide bioactivities.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Kiattawee Choowongkomon

Role: Thesis Advisor

Name: Orathai Sawatdichaikul

Role: Thesis Co-Advisor

Created: 2019

Modified: 2025-06-29

Issued: 2025-06-29

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2562/thitima-pan-all.pdf

CallNumber: TX553.P7 .T54

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Genetic Engineering

Grantor: Kasetsart University

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