Preparation and primary bioactivity evaluation of novel water-soluble curcumin-loaded chitooligosaccharides nanoformulation

Name: Pattarachat Ingrungruengluet

LCSH: Kasetsart University -- Theses. M.S. (Fishery Science and Technology) 2023.

Classification :.LCCS: QK495.Z65

LCSH: Kasetsart University. -- Faculty of Fisheries -- Theses.

LCSH: Turmeric.

LCSH: Curcumin.

Abstract: Curcumin (CU) is a yellow-bioactive compound extracted from turmeric, a polyphenolic compound. CU has various advantages in the pharmaceutical field; however, the benefits of CU are limited by its low water solubility (11 ng/mL). This research aimed to improve the physical and biological activity of curcumin which fabricated a water-soluble CU nano-formulation with chitooligosaccharides (COS) and pluronic F-68 (PF) by utilizing the polymeric micelle preparation method. Firstly, the optimized curcumin-loaded chitooligosaccharides/pluronic F-68 micelles (COSPFCU) exhibited high encapsulation efficiency and loading capacity at the preparation temperature of 80 °C and 0.5% w/v of PF (75.57 ± 2.35% and 10.32 ± 0.59%, respectively). Secondly, the particle size of COSPFCU was determined by dynamic scattering analysis, which showed the hydrodynamic diameter at 73.89 ± 11.69 nm with a polydispersity index below 0.3 and 9.89 ± 0.21 mV of zeta potential. The Fourier transform infrared spectroscopy (FTIR) and Proton nuclear magnetic resonance spectroscopy (1H NMR) results confirmed the formation of particles with noncovalent bonds. Thirdly, the COSPFCU could be completely redispersed in water, which showed high antioxidant activity with a scavenging activity of 80.49% at 0.5 mg/mL (0.05 mg/mL of CU, 10% w/w in COSPFCU), comparable to 0.05 mg/mL of CU in ethanol with a scavenging activity of 88.73%. Meanwhile, COSPFCU could significantly reduce the cytotoxicity of the RAW 264.7 cells compared to native CU. Furthermore, COSPFCU illustrated anti-inflammatory activity, with the inhibition of nitric oxide (NO) production in lipopolysaccharide stimulation RAW 264.7 cells at 72.83 ± 2.37% and 33.20 ± 3.41% for the CU, with a low cytotoxicity to the macrophage cells. In conclusion, these results confirmed the potential of the COS-PF polymeric micelles system that could improve the physical and biological properties of CU, moreover, these particles could delay their release for a sustainable environment.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Yaowapha Waiprib

Role: Thesis Advisor

Name: Li Chunxia

Role: Thesis Co-Advisor

Created: 2023

Modified: 2025-06-24

Issued: 2025-06-24

วิทยานิพนธ์/Thesis

วิทยานิพนธ์/Thesis

application/pdf

URL: http://www.lib.ku.ac.th/KUthesis/2566/pattarachat-ingr-all.pdf

CallNumber: QK495.Z65.P38

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Fishery Science and Technology

Grantor: Kasetsart University

©copyrights Kasetsart University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	pattarachat-ingr-all.pdf	1.88 MB

ใช้เวลา

0.03952 วินาที