Evaluation of total phenolic contents and in-vitro antioxidant activity of hemp leaves extracts for its application in cosmetic product

Name: Nutlada Nusontra

Organization : Mae Fah Luang University. School of Science

Name: Chakree Wattanasiri

Organization : Mae Fah Luang University. School of Integrative Medicine

Name: Naphatsorn Ditthawutthikul

Organization : Mae Fah Luang University. School of cosmetic science

Name: Acharavadee Bunkoom

Organization : Mae Fah Luang University. School of Science

Email : acharavadee.pan@mfu.ac.th

LCSH: Hemp -- Analysis

LCSH: Phenols

LCSH: Antioxidants -- Analysis

LCSH: Plant extracts

Abstract: Hemp (Cannabis sativa L.) contains cannabidiol (CBD) with lower amount of Δ9-tetrahydrocannabinol compared to cannabis. It also contains terpenoid, flavonoids, and other. These phytochemical compounds have therapeutic effects. Hemp has been utilized for various purposes, including ingredients in cosmetics formulations. Hemp leaves are the main waste of industrial hemp plantation. This study aimed to produce ethanolic extract of hemp leaves using a plant to solvent ratio of 1:10. Ethanolic extract of hemp leaves was investigated and evaluated for it in-vitro biological activity focusing on antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging capacity and hydrogen peroxide (H2O2) scavenging method. In addition, total phenolic content expressed as total flavonoids (TFC) content and total tannin content (TTC) of the extracts were studied. The flavonoids total and total tannin amount were analyzed by Folin-Ciocalteu’s assay and aluminum chloride colorimetry, respectively. The result showed that hemp extract showed antioxidant activity with the IC50 value in DPPH scavenging assay at 0.32±0.05 mg/mL, while Trolox (standard) showed at IC50 3.04±0.05 μg/mL. The H2O2 scavenging method showed the hemp extracts IC50 value at 0.258±0.034 mg/mL while ascorbic acid (standard) showed 0.131±0.006 mg/mL. Flavonoids content and tannic content were found at 129.90±14.73 mg quercetin equivalent/g and 65.12±9.89 mg tannic acid equivalent/g extract, respectively. Phenolic compounds influence antioxidant activity that can minimize damages of skin cells from oxidative stress. All these results suggested that ethanolic hemp leaves extract can be used as active ingredients in skin care products and use as an alternative source of antioxidant compounds in the cosmetics industry.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2024

Modified: 2024-12-02

Issued: 2024-12-02

บทความ/Article

application/pdf

BibliograpyCitation : In Kasetsart University. Faculty of Science. The Pure and Applied Chemistry International Conference 2024 (PACCON 2024) (pp.491-495). Bangkok : Kasetsart University, 2024

eng

©copyrights King Mongkut's University of Technology North Bangkok

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	PACCON 2024pp.491-495.pdf	472.86 KB

ใช้เวลา

0.036284 วินาที