Determination of the 9-tetrahydrocannabinol metabolite in urine by online-solid phase extraction coupled with liquid chromatography-mass spectrometry

Name: Waranyu Nachiangtai

Organization : Mahasarakham University. Faculty of Science

Name: Ajjima Thongbo

Organization : Regional Medical Sciences Center 8 Udon Thani

Name: Chuleeporn Jantarasena

Organization : Regional Medical Sciences Center 8 Udon Thani

Name: Sittiporn Parnmem

Organization : Ministry of Public Health. Department of Medical Sciences

Name: Kraingkrai Ponhong

Organization : Mahasarakham University. Faculty of Science

Email : kraingkrai.p@msu.ac.th

LCSH: Cannabinoids -- Analysis

LCSH: Tetrahydrocannabinol -- Detection

LCSH: Mass spectrometry

Abstract: Delta-9-tetrahydrocannabinol (THC), the bioactive compound in marijuana (Cannabis sativa L.), is used for medical purposes at low concentrations. THC is metabolized in the liver and transformed to 11-nor- Δ9-tetrahydrocannabinol-9-carboxylic acid (11-nor-9-THC-COOH), which is excreted in the urine. This study developed a sensitive analytical method for the determination of the THC metabolite in urine at trace levels. Online-solid phase extraction (HyperSep Retain PEP Online-SPE column) was employed to extract 11-nor-9-THC-COOH in urine before analysis by liquid chromatography-mass spectrometry (LC-MS). Online-SPE is a simple and rapid method that reduces preparation steps by passing the sample through an SPE cartridge and then eluting with an appropriate solvent before separating by LC-MS. This method achieved detection and quantification limits at 0.20 ng/mL and 1.00 ng/mL, respectively with linearity range 1.00– 100.00 ng/mL. The accuracy value (%bias) and precision value of the developed method were within the SWGTOX guidelines (±20%), with percentage recovery 99.16–101.60%. Therefore, this method is accurate, precise, and appropriate for analyzing 11-nor-9-THC-COOH metabolite in urine samples at low-level concentrations

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2024

Modified: 2024-11-26

Issued: 2024-11-26

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BibliograpyCitation : In Kasetsart University. Faculty of Science. The Pure and Applied Chemistry International Conference 2024 (PACCON 2024) (pp.121-127). Bangkok : Kasetsart University, 2024

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