Development of colorimetric methods for determination of total phenolic content in tea

Name: Phackjira Prasitthirotjanayut

Organization : Maejo University. Faculty of Science

Name: Yanwisa Rodma

Organization : Maejo University. Faculty of Science

Name: Kelly M. Boonloed

Organization : Maejo University. Faculty of Science

Name: Anukul Boonloed

Organization : Maejo University. Faculty of Science, Maejo University. Center of Excellence in Agricultural Innovation for Graduate Entrepreneur

Email : anukul.boonloed@gmail.com

ThaSH: Tea -- Analysis

ThaSH: Colorimetric analysis

ThaSH: Analytical chemistry

ThaSH: Phenols

ThaSH: Plant extracts

Abstract: In this work, a colorimetric method has been developed utilizing a smartphone and a home-office scanner to determine total phenolic content of three types of commercially available teas. The colorimetric determination relied on the measurement of the aqueous blue product yielded from the Folin-Ciocalteu phenolic assay. The smartphone device layout consisted of a light box and an iPhone 7 plus. The images were processed using the application, ColorAssist. The smartphone results yielded a total phenolic content for Baimon tea, Taiwan tea, and Black tea of 3.78, 23.83, and 40.91 mg GAE/g tea, respectively. The scanner device layout consisted of an office scanner and a 96-well microplate. The images were processed using ImageJ. The scanner results gave a total phenolic content for Baimon tea, Taiwan tea, and Black tea of 5.55, 15.27, and 42.27 mg GAE/g tea, respectively. The results obtained from the colorimetric methods were comparable to those from the spectrophotometric plate reader used as a traditional method. The total phenolic content obtained from the spectrophotometric microplate method for Baimon tea, Taiwan tea, and Black tea were 4.65, 20.83, and 36.35 mg GAE/g tea, respectively. These methods can provide convenient, fast and efficient measurement compared to traditional methods.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2020

Modified: 2024-10-09

Issued: 2024-10-09

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BibliograpyCitation : In the Chemical Society of Thailand under the Patronage of Professor Dr. HRH Princess Chulabhorn in association, Thammasat University Faculty of Science and Technology. The Pure and Applied Chemistry International Conference 2020 (PACCON 2020) (FA101-105). Bangkok : Thammasat University

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