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Sirinan Thubthimthed. Amino acids profiling, cytotoxicity test and nitric oxide inhibition of gelatin extracted from jelly fish (Lobonema smithii) derived from local fishery. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2020.
Amino acids profiling, cytotoxicity test and nitric oxide inhibition of gelatin extracted from jelly fish (Lobonema smithii) derived from local fishery
Organization :
Thailand Institute of Scientific and Technological Research. Expert Centre of Innovative Herbal products
Email :
khanitha@tistr.or.th
Organization :
Thailand Institute of Scientific and Technological Research. Expert Centre of Innovative Herbal products
Organization :
Thailand Institute of Scientific and Technological Research. Expert Centre of Innovative Herbal products
Name: Jarussang Kammultri
Organization :
Thailand Institute of Scientific and Technological Research. Industrial Metrology and Testing service Centre
Name: Praphakorn Kaemchanteuk
Organization :
Thailand Institute of Scientific and Technological Research. Expert Centre of Innovative Herbal products
Name: Sirinan Thubthimthed
Organization :
Thailand Institute of Scientific and Technological Research. Expert Centre of Innovative Herbal products
keyword:
Lobonema smithii
ThaSH:
Jellyfishes
-- Immunology
ThaSH:
Amino acids
-- Derivatives
ThaSH:
Toxicity testing
Abstract:
Gelatin, a fibrous protein from degradation of collagen from skin and bone of pig and cow, unacceptable for the Halal and Kosher food markets. The occurrence of Bovine Spongiform Encephalopathy (BSE) caused anxiety of bovine gelatin. This study aimed to extract gelatins from jelly fish and evaluate their amino acids, cytotoxicity and nitric oxide inhibition. The jelly fish (Lobonema smithii) in the form of fresh, fermented and residues from industrial jelly fish process were extracted using aqueous alkaline, pH 14, 4 degrees for 6 hrs., the heating at 60 degrees and 75 degrees, for 6 hrs and 12 hrs after pH7 adjustment. The extracts were analysed for 18 amino acids, 1H NMR, cytotoxicity by MTT assay and nitric oxide inhibition by using RAW 264.7 cell line. The results showed the highest percent yield was the gelatin from fermented jelly fish. The heat process presented nearly double yield to the 4 degrees extraction whereas the gelatin from residues resulted in the highest amino acids. Few different gelatin percentages were found from 6 and 12 hrs extraction. The gelatin from fresh one exhibited cytotoxicity, IC50>5 mg/mL, and the nitric oxide inhibition, 1.14+1.10, 2.34±1.60 and 47.17+3.17 at the concentration of 1.25, 2.5 and 5 mg/mL, respectively. The other gelatins will be further tested for their in vitvo anti-inflammation and will focus on immuno-stimulant of the active gelatin.
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2020
Modified:
2024-10-08
Issued:
2024-10-08
บทความ/Article
application/pdf
BibliograpyCitation :
In the Chemical Society of Thailand under the Patronage of Professor Dr. HRH Princess Chulabhorn in association, Thammasat University Faculty of Science and Technology. The Pure and Applied Chemistry International Conference 2020 (PACCON 2020) (FA20-24). Bangkok : Thammasat University
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.051545 วินาที
Khanittha Chawananorasest
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มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kittirat Prangchan;Khanittha Chawananorasest;Chaweewan Poonthananiwatkul;Ghit Laungsopapun;Singto Sakulkhaemaruethai | บทความ/Article | |
| Amino acids profiling, cytotoxicity test and nitric oxide inhibition of gelatin extracted from jelly fish (Lobonema smithii) derived from local fishery
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Khanittha Chawananorasest;Sarunya Laovitthayanggoon;Pattarawadee Kengkwasingh;Jarussang Kammultri;Praphakorn Kaemchanteuk;Sirinan Thubthimthed | บทความ/Article |
Sarunya Laovitthayanggoon
| Title | Contributor | Type |
|---|---|---|
| Amino acids profiling, cytotoxicity test and nitric oxide inhibition of gelatin extracted from jelly fish (Lobonema smithii) derived from local fishery
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Khanittha Chawananorasest;Sarunya Laovitthayanggoon;Pattarawadee Kengkwasingh;Jarussang Kammultri;Praphakorn Kaemchanteuk;Sirinan Thubthimthed | บทความ/Article |
Pattarawadee Kengkwasingh
| Title | Contributor | Type |
|---|---|---|
| Amino acids profiling, cytotoxicity test and nitric oxide inhibition of gelatin extracted from jelly fish (Lobonema smithii) derived from local fishery
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Khanittha Chawananorasest;Sarunya Laovitthayanggoon;Pattarawadee Kengkwasingh;Jarussang Kammultri;Praphakorn Kaemchanteuk;Sirinan Thubthimthed | บทความ/Article |
Jarussang Kammultri
| Title | Contributor | Type |
|---|---|---|
| Amino acids profiling, cytotoxicity test and nitric oxide inhibition of gelatin extracted from jelly fish (Lobonema smithii) derived from local fishery
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Khanittha Chawananorasest;Sarunya Laovitthayanggoon;Pattarawadee Kengkwasingh;Jarussang Kammultri;Praphakorn Kaemchanteuk;Sirinan Thubthimthed | บทความ/Article |
Praphakorn Kaemchanteuk
| Title | Contributor | Type |
|---|---|---|
| Amino acids profiling, cytotoxicity test and nitric oxide inhibition of gelatin extracted from jelly fish (Lobonema smithii) derived from local fishery
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Khanittha Chawananorasest;Sarunya Laovitthayanggoon;Pattarawadee Kengkwasingh;Jarussang Kammultri;Praphakorn Kaemchanteuk;Sirinan Thubthimthed | บทความ/Article |
Sirinan Thubthimthed
| Title | Contributor | Type |
|---|---|---|
| Amino acids profiling, cytotoxicity test and nitric oxide inhibition of gelatin extracted from jelly fish (Lobonema smithii) derived from local fishery
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Khanittha Chawananorasest;Sarunya Laovitthayanggoon;Pattarawadee Kengkwasingh;Jarussang Kammultri;Praphakorn Kaemchanteuk;Sirinan Thubthimthed | บทความ/Article |