Chaisang Tamang. Physicochemical and antifungal properties of pyroligneous acid derived from macadamia nutshell and its application. Master's Degree(Postharvest Technology and Innovation). Mae Fah Luang University. Learning Resources and Educational Media Center. : Mae Fah Luang University, 2023.
Physicochemical and antifungal properties of pyroligneous acid derived from macadamia nutshell and its application
Abstract:
Pyroligneous acid (PA), resulting from the pyrolysis of lignocellulosic bio-masses, exhibits antioxidant, antimicrobial and insecticidal property. This study explores the physicochemical properties and antimicrobial efficacy of PA derived from macadamia nutshell (MNS). A comparative analysis between distillation-refined and naturally-refined PA was tested. Distillation-refined PA exhibited superior qualities, with a lower pH (2.65), higher total phenolic content (138.70 mg GAE/mL), and antioxidant capacity with IC50 value 7.27 mg mL-1) and stronger reducing power with 132.09 gM FeSO4/100mL compared to naturally-refined PA. In-vitro analysis demonstrated significant inhibition of Colletotrichum sps. mycelial growth at various PA concentrations, surpassing the fungicide Carbendazim. While 0.5% and 1% PA showed moderate inhibitory effects, higher concentrations were needed for complete inhibition of Lasiodiplodia theobromae and Aspergillus niger mycelium. Storage assessment of Nam Dok Mai Sithong mangoes reveal that PA (1.5%) combined with chitosan (1%) effectively maintains quality parameters over 20 days, with reduced disease incidence and severity. The combined treatment also exhibited the lowest disease incidence and severity scores. This comprehensive investigation underscores the potential of distillation-refined MNS-derived PA as a promising antimicrobial agent for mango preservation during storage.
Mae Fah Luang University. Learning Resources and Educational Media Center